The Miaohe-type Biota that are found from the upper part of Doushantuo Formation of Sinian at Taoying Town, Jiangkou County, northeastern Guizhou Province, South China includes macroalgal, possible metazoan, ichnofossil, and other fossils, and it was further more proved that the macrobiota was distributed widely in seafloor in Yangtze area, South China, during the Late Doushantuo Age of Sinian. Comparing with the Miaohe Biota in western Hubei Province and Lantian Flora in southern Anhui Province, we consider that the macrobiota mainly lived in the transitional area from the open platform to the platform edge slope facies. Studies on the Miaohe-type Biota in northeastern Guizhou, suggest that the life styles of macrobiota in the Late Doushantuo Age were mainly fixing on and attaching to the surface of seafloor, that the macrobiota lived in relatively calm-water shallow sea with abundant light, poor-oxygen, and a measure of water-energy, and that the ground macrobiota living on was the unfirmed soup-soft ground with rich water. During the Late Doushantuo Age, most of the macro-organisms were erect and semi-floating on the seafloor. They formed primitive “submarine grassland”, and produced oxygen to provide animals that depended on oxygen for living and reproducing. After these organisms were dead, their corpses laid on the surface of sediments and could be kept well in the environment with poor oxygen and fast sedimentation rate. Then they were buried rapidly by sediments. With the sediments being increased, these corpses were closed perfectly in the sediments to stop the decaying and decomposing process, and the macrobiota are preserved very well. The fast sedimentation rate, poor oxygen and comparatively calm-water environment are important factors making the biota be preserved well.