A highly diversified macrofossil assemblage was discovered from the Jiucheng Member of uppermost Ediacaran of Jinning, Jiangchuan area in eastern Yunnan. Besides abundant Vendotaenia and Tyrasotaenia, the big multicellular benthos such as Tawuia-like and Longfengshaniaceaen fossils with diverse holdfast structures, have distinct dominance, while there are still some other macrofossils with peculiar configurations as well as indistinct relatives. There is an obvious distinction between this assemblage and the macrofossil assemblages from the Doushantuo Formation and the Shibantan Member of Dengying in South China. This paper has outlined the characteristics of some of the multicellular fossils from the Jiucheng Member at Jiangchuan. They include some macrofossils with different types of holdfast structures, and indistinct affinities as well. The discovery of the diverse-shaped macrofossil assemblages from the Jiucheng Member ineastern Yunnan indicates the diversification and radiation of metaphytes during the latest Ediacaran Period. The contemporary radiations of phytoplankton and benthic metaphyte maybe provide important contribution to the Early Cambrian explosion of animals.