SPARC蛋白在胃癌中的表达及与胃癌预后的关系

doi:10.11904/j.issn.1002-3070.2015.06.005

实用肿瘤学杂志 ›› 2015, Vol. 29 ›› Issue (6): 501-506.doi: 10.11904/j.issn.1002-3070.2015.06.005

SPARC蛋白在胃癌中的表达及与胃癌预后的关系

王小霞¹, 杨明², 张艳桥²

1.哈尔滨医科大学附属第二医院急诊内科(哈尔滨 150086);
2.哈尔滨医科大学附属肿瘤医院消化内科

收稿日期:2015-06-13 出版日期:2015-12-28 发布日期:2015-12-28
通讯作者: 张艳桥,E-mail:yanqiaozhang@126.com E-mail:yanqiaozhang@126.com
作者简介:王小霞,女,(1984-),硕士,住院医师,从事消化系肿瘤的基础及临床方面的研究

Secreted protein acidic and rich in cysteine(SPARC)expression in gastric adenocarcinoma and its relationship with the prognosis of patients with of gastric cancer

WANG Xiaoxia¹, YANG Ming², ZHANG Yanqiao²

1.Department of Emergency,The Second Affiliated Hospital of Harbin Medical University,Harbin 150086,China;
2.Department of Digestive Oncology,The Affiliated Tumor Hospital of Harbin Medical University

Received:2015-06-13 Online:2015-12-28 Published:2015-12-28

摘要/Abstract

摘要： 目的探讨富含半胱氨酸(Cys)的酸性分泌蛋白(SPARC蛋白)在胃腺癌组织中的表达及与胃腺癌患者预后的关系。方法免疫组化法检测104例胃腺癌组织和30例癌旁组织中SPARC蛋白的表达差异,并应用统计学的方法分析SPARC蛋白与胃腺癌患者预后的关系。结果 SPARC蛋白在30例癌旁胃黏膜组织中阳性率为20%(6/30),且均为弱表达或无表达,在104例胃腺癌组织中阳性率为76.1%(79/104),二者差异有统计学意义(P＜0.01)。胃腺癌组织中,SPARC蛋白表达高低与淋巴结转移及组织分化程度呈正相关(P＜0.05);SPARC蛋白高表达的胃腺癌患者平均生存时间为27.4个月,明显低于低表达者的40.9个月(P＜0.05)。结论 SPARC蛋白在胃腺癌组织中高表达,且主要表达于胃腺癌细胞周围的间质中;其与淋巴结转移及组织分化程度显著相关;SPARC蛋白高表达的胃腺癌患者生存期短,预后差,但SPARC蛋白不是其独立的预后因素。

关键词: SPARC蛋白, 胃腺癌, 免疫组化

Abstract: Objective To study the expression of acid secreted proteins that are rich in cysteine protein(SPARC protein)in gastric adenocarcinoma tissue and its relationship with the prognosis of patients with gastric cancer.Methods In order to detect SPARC protein expression levels in gastric adenocarcinoma,104 primary gastric adenocarcinoma samples as well as 30 normal gastric tissues adjacent to carcinoma were studied by immunohistochemistry.Results The positive rate of SPARC protein in 30 cases of gastric mucosa adjacent to carcinoma is 20%,and with weak expression or no expression;The positive rate in 104 cases of gastric cancer tissues was 76.1%,the difference was significant(P＜0.01).The high expression rate of SPARC and lymph node metastasis in gastric adenocarcinoma were positively correlated(P＜0.01).The SPARC expression rate of well and moderate differentiation of gastric adenocarcinoma were significantly lower than that of the low differentiation(P＜0.05).The average survival time of gastric cancer patients with high SPARC expression was 27.4 months.However,the average survival time of the patients with low SPARC expression was 40.9 months.The difference was significant(P＜0.05).Conclusion SPARC protein is mainly high expressed in gastric cancer stromal tissue cells and fibroblasts.The expression level of SPARC protein is significantly associated with lymph node metastasis and tumor differentiation degree.The high expression of SPARC patients survival time is short and the prognosis is poor.However,SPARC protein is not an independent prognostic factor.

Key words: SPARC protein, Gastric adenocarcinoma, Immunohistochemistry

中图分类号:

R735.2

王小霞, 杨明, 张艳桥. SPARC蛋白在胃癌中的表达及与胃癌预后的关系[J]. 实用肿瘤学杂志, 2015, 29(6): 501-506.

WANG Xiaoxia, YANG Ming, ZHANG Yanqiao. Secreted protein acidic and rich in cysteine(SPARC)expression in gastric adenocarcinoma and its relationship with the prognosis of patients with of gastric cancer[J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(6): 501-506.

参考文献

1 杨明,张艳桥.胃癌靶向治疗进展[J].实用肿瘤学杂志,2010,4(6):570-573.
2 Peralta DV,Heidari Z,Dash S,et al.Hybrid paclitaxel and gold nanorod-loaded human serum albumin nanoparticles for simultaneous chemotherapeutic and photothermal therapy on 4T1 breast cancer cells[J].ACS Appl Mater Interfaces,2015,7(13):7101-7111.
3 Zhao ZS,Wang YY,Chu YQ,et al.SPARC is associated with gastric cancer progression and poor survival of patients[J].Clin Cancer Res,2010,1(6):260-268.
4 Brekken RA,Sage EH.SPARC,a matricellular protein:at the crossroads of cell-matrix communication[J].Matrix Biol,2001,19(4):816-827.
5 Xue LY,Zou SM,Zheng S,et al.Expressions of the γ2 chain of laminin-5 and secreted protein acidic and rich in cysteine in esophageal squamous cell carcinoma and their relation to prognosis[J].Chin J Cancer,2011,30(1):69-78.
6 Liang JF,Wang HK,Hong X,et al.Relationship and prognostic significance of SPARC and VEGF protein expression in colon cancer[J].J Exp Clin Cancer Res,2010,29(1):71-79.
7 Cheetham S,Tang MJ,Mesak F,et al.SPARC promoter hypermethylation in colorectal cancers can be reversed by 5-Aza-2′deoxycytidine to increase SPARC expression and improve therapy response[J].Br J Cancer,2008,98(6):1810-1819.
8 Tichet M,Prod′Homme V,Fenouille N,et al.Tumour-derived SPARC drives vascular permeability and extravasation through endothelial VCAM1 signalling to promote metastasis[J].Nat Commun,2015,30(6):6993-6998.
9 Chen ZY,Zhang JL,Yao HX,et al.Aberrant methylation of the SPARC gene promoter and its clinical implication in gastric cancer[J].Scientific Reports,2014,17(4):7035-7044.
10 Cheetham S,Tang MJ,Mesak F,et al.SPARC promoter hypermethylation in colorectal cancers can be reversed by 5-Aza-2′deoxycytidine to increase SPARC expression and improve therapy response[J].Br J Cancer,2008,98(1):1810-1819.
11 韩伟,杨迷玲,翟丽丽,等.胃癌SPARC、TGFβ1和Ki-67的表达及意义[J].临床与实验病理学杂志,2011,6(1):594-597.
12 王涛,郑永光,李玉民,等.胃癌与肿瘤微环境的研究进展[J].实用肿瘤学杂志,2014,28(6):561-564.
13 Winder T,Wilson PM,Yang D,et al.An individual coding polymorphism and the haplotype of the SPARC gene predict gastric cancer recurrence[J].The Pharmacogenomics Journal,2012,10(1):1635-1659.
14 Yin J,Chen G,Liu Y,et al.Downregulation of SPARC expression decreases gastric cancer cellular invasion and survival[J].Exp Clin Cancer Res,2010,2(4):29-59.
15 Wang L,Yang M,Shan L,et al.The role of SPARC protein expression in the progress of gastric cancer[J].Pathol Oncol Res,2012,18(6):697-702.

[1]	张云艳, 刘亚琴, 王磊, 张琳, 成海燕, 杨姗姗. RIZ1蛋白表达与宫颈鳞状细胞癌临床病理参数及放疗敏感性的相关性研究[J]. 实用肿瘤学杂志, 2017, 31(5): 405-410.
[2]	于歌, 孙佳宁, 冯晓玲. 上皮性卵巢肿瘤组织中PSMD10、Ki67的表达及其临床病理意义[J]. 实用肿瘤学杂志, 2017, 31(2): 112-116.
[3]	毛维, 陈青娟, 司小敏, 张消, 蒋成. 分泌超高血清AFP胃肝样腺癌1例并文献复习[J]. 实用肿瘤学杂志, 2017, 31(1): 39-42.
[4]	刘静蕾, 姜季, 张明岩, 王影, 尤艳, 王艳. OLC1在结直肠癌中的表达及对预后影响的研究[J]. 实用肿瘤学杂志, 2015, 29(5): 390-394.
[5]	郑国良, 郑志超, 赵岩, 张涛, 张剑军. 胃癌HER-2表达的临床意义及血管新生相关性研究[J]. 实用肿瘤学杂志, 2015, 29(5): 438-443.
[6]	车爱文, 陈淑苹, 谭旭东, 李家梁, 马红梅. 宫颈癌组织中CIP2A和HPV16-E7的表达及其临床意义分析[J]. 实用肿瘤学杂志, 2015, 29(1): 29-33.
[7]	徐睿瑞, 温荔媛, 孙国强, 孟庆威, 孙立春, 蔡莉. Caveolin-1的表达与EGFR突变在NSCLC中的相关性及其临床意义的研究[J]. 实用肿瘤学杂志, 2014, 28(6): 498-502.
[8]	孙颖,田云霄,王双海,王静,宋晓明,张小辉. C-met和COX-2在甲状腺乳头状癌中的表达及其相关性研究[J]. 实用肿瘤学杂志, 2014, 28(5): 396-400.
[9]	刘运铎,孟凡玲,徐冶,杨萌. AEG-1、DLL4在子宫内膜癌中的表达及临床病理相关性的研究[J]. 实用肿瘤学杂志, 2013, 27(3): 223-228.
[10]	林梅,夏百荣,高舒. MTDH和VEGF-C在上皮性卵巢癌组织中的表达及其临床病理特征[J]. 实用肿瘤学杂志, 2013, 27(3): 229-233.
[11]	乌肖林, 耿敬姝. HMGA2、Smad4 在胃腺癌组织中的表达及其与上皮间质转化的相关性[J]. 实用肿瘤学杂志, 2013, 27(1): 26-30.
[12]	樊秋兰, 董晓秋, 邵小慧, 王璐璐, 张立维. 乳腺癌超声特征与Ki-67表达的相关性[J]. 实用肿瘤学杂志, 2013, 27(1): 65-68.
[13]	杜春, 祁紫娟, 耿敬姝. HMGA2、Twist在胃腺癌中的表达及其与EMT的关系[J]. 实用肿瘤学杂志, 2012, 26(6): 495-500.
[14]	吕威, 冯美燕, 宋鸿涛, 姜影, 佟丹丹, 李玉莲, 石清涛, 耿敬姝. AKR1C2与Twist在胃组织中的表达及其临床病理相关性的研究[J]. 实用肿瘤学杂志, 2012, 26(5): 389-393.
[15]	王鹏, 张艳枝, 石穆穆, 李淑芝, 李玉荣, 朱辉. 我国北方汉族女性乳腺癌中PELP1/MNAR表达特征及其与Ki-67标记指数相关性研究[J]. 实用肿瘤学杂志, 2012, 26(5): 452-457.

SPARC蛋白在胃癌中的表达及与胃癌预后的关系

Secreted protein acidic and rich in cysteine(SPARC)expression in gastric adenocarcinoma and its relationship with the prognosis of patients with of gastric cancer

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价