MicroRNA-204对胶质瘤细胞U251自噬的影响

doi:10.11904/j.issn.1002-3070.2016.01.005

实用肿瘤学杂志 ›› 2016, Vol. 30 ›› Issue (1): 23-27.doi: 10.11904/j.issn.1002-3070.2016.01.005

MicroRNA-204对胶质瘤细胞U251自噬的影响

付爽¹, 刘国军²

1.哈尔滨医科大学附属肿瘤医院(哈尔滨 150081);
2.农垦总医院康复科

收稿日期:2015-09-26 出版日期:2016-02-28 发布日期:2016-02-25
通讯作者: 付爽,E-mail:13704817946@163.com
作者简介:付爽,女,(1985-),硕士,住院医师,从事肿瘤内科和神经科的研究

The effect of microRNA-204 on autophagy in U251

FU Shuang¹, LIU Guojun²

1.Deparment of General Medicine,The Affiliated Tumor Hospital of Harbin Medical University,Harbin 150081,China;
2.Department of Rehabilitation,Land Reclamation General Hospital

Received:2015-09-26 Online:2016-02-28 Published:2016-02-25

摘要/Abstract

摘要： 目的探讨microRNA-204(miR-204)对胶质瘤细胞U251自噬的影响。方法采用体外瞬时转染的方法抑制U251细胞中miR-204,MTT方法检测细胞的增殖情况,免疫荧光检测细胞自噬,Western blot检测自噬相关蛋白LC3、Beclin1和Bcl-2的表达情况。结果 MTT结果显示抑制miR-204明显增加U251细胞的增殖,免疫荧光结果显示抑制miR-204能够减少U251细胞的自噬,Western blot结果显示抑制miR-204能够减少U251细胞内LC3-Ⅱ和Beclin1的表达量,然而Bcl-2的表达却明显增加。结论 miR-204可能通过上调Bcl-2的表达抑制自噬促进胶质瘤生长,提示miR-204可能成为脑胶质瘤潜在治疗靶点。

关键词: 胶质瘤, 微小RNA-204, Bcl-2, 自噬

Abstract: Objective To investigate the effect of microRNA-204(miR-204)on autophagy in U251.Methods Inhibition of miR-204 in U251 cell lines was done by transfection of miR-204 inhibitor(AMO-204).Cell viability was detected by MTT assay.The autophagy of U251 was tested by immunofluorescence technique.The protein level of Beclin 1,LC3 and Bcl-2 was detected by Western blot.Results Cell viability was markedly increased after inhibition of miR-204 in U251 cells.The number of autophagosome was decreased.The levels of Beclin 1 and LC3 were decreased,the protein level of Bcl-2 was significant increased by transfection of AMO-204 in U251 cells(P＜0.05).Conclusion MiR-204 might at least in part promote glioma via inhibiton autophagy,indicating that miR-204 might be a potential target for the treatment of glioma.

Key words: Glioma, MicroRNA-204, Bcl-2, Autophagy

中图分类号:

R739.41

付爽, 刘国军. MicroRNA-204对胶质瘤细胞U251自噬的影响[J]. 实用肿瘤学杂志, 2016, 30(1): 23-27.

FU Shuang, LIU Guojun. The effect of microRNA-204 on autophagy in U251[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(1): 23-27.

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MicroRNA-204对胶质瘤细胞U251自噬的影响

The effect of microRNA-204 on autophagy in U251

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