癌性腹水对卵巢癌细胞增殖及迁移的影响

doi:10.11904/j.issn.1002-3070.2016.01.007

实用肿瘤学杂志 ›› 2016, Vol. 30 ›› Issue (1): 36-40.doi: 10.11904/j.issn.1002-3070.2016.01.007

癌性腹水对卵巢癌细胞增殖及迁移的影响

郭爽^1,2, 刘端阳¹, 李静¹, 李佳德¹, 金晓明¹

1.哈尔滨医科大学(哈尔滨 150086);
2.哈尔滨市疾病预防控制中心

收稿日期:2015-10-26 出版日期:2016-02-28 发布日期:2016-02-25
通讯作者: 金晓明,E-mail:1060774188@qq.com
作者简介:郭爽,女,(1987-),硕士,助理医师,从事卵巢癌细胞行为学特征的相关研究

The effect of malignant ascites on the proliferation and migration of ovarian cancer cells

GUO Shuang^1,2, LIU Duanyang¹, LI Jing¹, LI Jiade¹, JIN Xiaoming¹

1.Harbin Medical University,Harbin 150086,China;
2.Harbin Center for Disease Control and Prevention

Received:2015-10-26 Online:2016-02-28 Published:2016-02-25

摘要/Abstract

摘要： 目的探讨癌性腹水对卵巢癌腹水中肿瘤细胞和卵巢癌细胞系(SKOV3)的形态学特征、增殖和迁移能力的影响。方法卵巢癌腹水中提取的肿瘤细胞在体外用DMEM高糖培养液培养;卵巢癌细胞系(SKOV3)分别用DMEM高糖培养液和DMEM高糖培养液中加入不同比例癌性腹水制成的混合培养液培养;分别通过光学显微镜和电子显微镜观察各组细胞的形态学特点;用CCK试剂盒检测各组细胞的增殖能力;用划痕实验检测癌性腹水对卵巢癌细胞系SKOV3迁移能力的影响。结果卵巢癌腹水中肿瘤细胞与卵巢癌细胞系(SKOV3)形态学特征明显不同;腹水中的肿瘤细胞离开腹水微环境后增殖能力明显减弱;混合培养液培养的SKOV3肿瘤细胞比DMEM高糖培养液培养的SKOV3肿瘤细胞的增殖及迁移能力明显增强。结论卵巢癌腹水中的肿瘤细胞发生了更有利于其增殖及迁移的形态学改变;癌性腹水对卵巢癌细胞系(SKOV3)的增殖及迁移具有促进作用。

关键词: 卵巢癌, 癌性腹水, 卵巢癌细胞系(SKOV3), 增殖能力, 迁移能力

Abstract: Objective To evaluate the effects of malignant ascites on the morphological characteristics and the proliferation and migration abilities of the tumor cell and ovarian cancer cell lines(SKOV3)in the ovarian cancerous ascites.Methods Tumor cells extracted from the ovarian cancerous ascites were cultured in vitro with DMEM high glucose culture medium,and ovarian cancer cell lines(SKOV3)were cultured in DMEM high glucose and DMEM high glucose with different proportion of malignant ascites.The morphological characteristics of the cells were observed by optical microscope and electron microscope respectively.Cell proliferation ability was detected by CCK kit;The effect of SKOV3 on the migration of ovarian cancer cell lines was measured by scratch test.Results The morphological characteristics of tumor cells and ovarian cancer cell lines(SKOV3)in ovarian cancer ascites were significantly different.The proliferation ability of tumor cells was decreased without the ascites.The proliferation and migration abilities of SKOV3 cultured in mixed culture medium were significantly improved compared with the cells cultured in high glucose medium.Conclusion The change of tumor cell morphology in ascites benefits its abilities of proliferation and migration.The malignant ascites promote the abilities of proliferation and migration of ovarian cancer cell line(SKOV3).

Key words: Ovarian cancer, Cancerous ascites, Ovarian cancer cell line(SKOV3), Proliferation, Migration

中图分类号:

R737.31

郭爽, 刘端阳, 李静, 李佳德, 金晓明. 癌性腹水对卵巢癌细胞增殖及迁移的影响[J]. 实用肿瘤学杂志, 2016, 30(1): 36-40.

GUO Shuang, LIU Duanyang, LI Jing, LI Jiade, JIN Xiaoming. The effect of malignant ascites on the proliferation and migration of ovarian cancer cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(1): 36-40.

参考文献

1 Harding V,Fenu E,Medani H,et al.Safety,cost-effectiveness and feasibility of daycase paracentesis in the management of malignant ascites with a focus on ovarian cancer[J].Br J Cancer,2012,107(6):925-930.
2 Sung SY,Hsieh CL,Wu D,et al.Tumor microenvironment promotes cancer progression,metastasis,and therapeutic resistance[J].Curr Probl Cancer,2007,31(2):36-100.
3 Emens LA,Silverstein SC,Khleif S,et al.Toward integrative cancer immunotherapy:targeting the tumor microenvironment[J].J Transl Med,2012,10(129):70-78.
4 Valentina C,Gloria B,Ubaldo E,et al.Metabolic remodeling of the tumor microenvironment migration stimulating factor(MSF)reprograms myofibroblasts toward lactate production,fueling anabolic tumor growth[J].Cell Cycle,2012,11(18):3403-3414.
5 Sada E,Follana P,Peyrade F,et al.Pathogenesis and management of refractory malignant ascites[J].Bull Cancer,2011,98(6):679-687.
6 Becker G,Galandi D,Blum HE.Malignant ascites:systematic review and guideline for treatment[J].Eur J Cancer,2006,42(5):589-597.
7 Kipps E,Tan DS,Kaye SB.Meeting the challenge of ascites in ovarian cancer:new avenues for therapy and research[J].Nat Rev Cancer,2013,13(4):273-282.
8 Jiang L,Liu R,Wang Y,et al.The role of Cyclin G1 in cellular proliferation and apoptosis of human epithelial ovarian cancer[J].J Mol Histol,2015,46(3):291-302.
9 Celik B,Didem Yalcin A,Esra Genc G,et.al.Proteomics pattern of peritoneal sApo-2L but not CD200(OX-2)as a possible screening biomarker for metastatic ovarian,endometrial and breast carcinoma[J].J Buon,2015,20(1):280-286.
10 Ip CK,Yung S,Chan TM,et al.p70 S6 kinase drives ovarian cancer metastasis through multicellular spheroid-peritoneum interaction and P-cadherin/b1 integrin signaling activation[J].Oncotarget,2014,5(19):9133-9149.
11 Latifi A,Luwor RB,Bilandzic M,et al.Isolation and characterization of tumor cells from the ascites of ovarian cancer patients:molecular phenotype of chemoresistant ovarian tumors[J].PLoS One,2012,7(10):e46858.
12 Jelovac D,Armstrong DK.Recent progress in the diagnosis and treatment of ovarian cancer[J].CA Cancer J Clin,2011,61(3):183-203.
13 Puiffe ML,Le Page C,Filali-Mouhim A,et al.Characterization of ovarian cancer ascites on cell invasion,proliferation,spheroid formation,and gene expression in an in vitro model of epithelial ovarian cancer[J].Neoplasia,2007,9(10):820-829.

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癌性腹水对卵巢癌细胞增殖及迁移的影响

The effect of malignant ascites on the proliferation and migration of ovarian cancer cells

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