过表达von Hippel-Lindau蛋白抑制小鼠结肠癌的生长及肝转移的研究

doi:10.11904/j.issn.1002-3070.2016.05.001

实用肿瘤学杂志 ›› 2016, Vol. 30 ›› Issue (5): 385-390.doi: 10.11904/j.issn.1002-3070.2016.05.001

• 论著 • 下一篇

过表达von Hippel-Lindau蛋白抑制小鼠结肠癌的生长及肝转移的研究

苗绘¹, 朱龙图², 许芝林¹, 赵铮¹, 王继洲²

1.哈尔滨医科大学附属第一医院小儿外科(哈尔滨 150081);
2.哈尔滨医科大学附属第一医院肝脏外科

收稿日期:2016-07-06 出版日期:2016-10-28 发布日期:2016-11-02
通讯作者: 王继洲,E-mail:wangjizhou1984@hotmail.com
作者简介:苗绘,女,(1985-),硕士,住院医师,从事小儿肝脏肿瘤、肝纤维化综合治疗的研究
基金资助:
国家自然科学基金(81200304);中国博士后科学基金(2012M520770);黑龙江省自然科学基金(QC2012C112);黑龙江省博士后科学基金(LBH-Z12162);哈尔滨医科大学附属第一医院科研基金(2012BS005)

Overexpression of von Hippel-Lindau protein inhibits the growth of colon cancer and liver metastasis in mice

MIAO Hui¹, ZHU Longtu², XU Zhilin¹, ZHAO Zheng¹, WANG Jizhou²

1.Department of Pediatric Surgery,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,China;
2.Department of Hepatic Surgery,The First Affiliated Hospital of Harbin Medical University

Received:2016-07-06 Online:2016-10-28 Published:2016-11-02

摘要/Abstract

摘要： 目的本研究拟探索在体内和体外过表达VHL蛋白能否抑制结肠癌的生长和转移。方法应用含有VHL的腺病毒在体外及体内过表达VHL蛋白。在体外检测结肠癌细胞CT26的增殖、凋亡和迁移,应用Western blot检测相关蛋白的表达。建立结肠癌皮下移植瘤和肝转移癌模型并判断治疗效果。结果过表达VHL蛋白通过调控COX-2、Bcl-2和MMP-9的表达抑制结肠癌细胞CT26增殖和侵袭,促进CT26细胞凋亡。在体内过表达VHL蛋白可抑制结肠癌皮下移植瘤的增长和结肠癌的肝转移。结论过表达VHL蛋白可抑制结肠癌的增长和转移,促进结肠癌细胞凋亡,提示VHL可能作为结肠癌治疗的一个潜在靶点。

关键词: von Hippel-Lindau蛋白, 结肠癌, 肝转移

Abstract: Objective To investigate whether overexpression of VHL can inhibit the growth and metastasis of colon cancer.Methods Adenovirus vectors containing VHL were used to overexpress VHL protein in vitro and in vivo.The proliferation,apoptosis and migration of colon cancer cells CT26 were examined in vitro.The expressions of related proteins were detected by Western blot analysis.Subcutaneous and liver metastasis of colon cancer models were established and the therapeutic effects were evaluated.Results Overexpression of VHL protein inhibited the proliferation and invasion of CT26,and promoted the apoptosis of CT26 via the regulation of COX-2,Bcl-2 and MMP-9.Overexpression of VHL protein inhibited the growth of subcutaneous colon cancer and liver metastasis in vivo.Conclusion Overexpression of VHL protien can inhibit the growth and metastasis of colon cancer,and enhance the apoptosis of colon cancer,indicating that VHL can be used as a potential target for colon cancer therapy.

Key words: von Hippel-Lindau protein, Colon cancer, Liver metastasis

中图分类号:

R735.3⁺5

苗绘, 朱龙图, 许芝林, 赵铮, 王继洲. 过表达von Hippel-Lindau蛋白抑制小鼠结肠癌的生长及肝转移的研究[J]. 实用肿瘤学杂志, 2016, 30(5): 385-390.

MIAO Hui, ZHU Longtu, XU Zhilin, ZHAO Zheng, WANG Jizhou. Overexpression of von Hippel-Lindau protein inhibits the growth of colon cancer and liver metastasis in mice[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(5): 385-390.

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过表达von Hippel-Lindau蛋白抑制小鼠结肠癌的生长及肝转移的研究

Overexpression of von Hippel-Lindau protein inhibits the growth of colon cancer and liver metastasis in mice

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