乙肝病毒蛋白和基因在肝内和肝外胆管癌组织中的检测及意义

doi:10.11904/j.issn.1002-3070.2017.01.002

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (1): 7-12.doi: 10.11904/j.issn.1002-3070.2017.01.002

乙肝病毒蛋白和基因在肝内和肝外胆管癌组织中的检测及意义

吴旖琦¹, 刘颖², 赵丽³, 金晓明¹

1.哈尔滨医科大学基础医学院病理教研室(哈尔滨 150081);
2.黑龙江省医院烧伤科;
3.哈尔滨市儿童医院重症医学科

收稿日期:2016-09-19 出版日期:2017-02-28 发布日期:2017-02-20
通讯作者: 金晓明,E-mail:jinxm55@163.com
作者简介:吴旖琦,女,(1977-),博士,副教授,从事消化系统肿瘤的研究
基金资助:
黑龙江省教育厅科学技术研究面上项目(12541361)

Detection of hepatitis B virus protein and gene in intrahepatic and extrahepatic cholangiocarcinoma tissue

WU Yiqi¹, LUI Ying², ZHAO Li³, JIN Xiaoming¹

1.Department of Pathology,Basic Medical Science College,Harbin Medical University,Harbin 150081,China;
2.Department of Burn,Heilongjiang Province Hospital;
3.Intensive Medicine,Harbin Children's Hospital

Received:2016-09-19 Online:2017-02-28 Published:2017-02-20

摘要/Abstract

摘要：

目的研究乙型肝炎病毒(Hepatitis B virus,HBV)感染与胆管癌(Cholangiocarcinoma,CC)的关系,并明确其与肝内胆管癌(Intrahepatic cholangiocarcinoma,ICC)和肝外胆管癌(Extrahepatic cholangiocarcinoma,ECC)发生的关系。方法采用免疫组化、巢式PCR对52例CC(21例肝内胆管癌和31例肝外胆管癌)石蜡包埋组织进行HBV相关蛋白及基因的检测。结果 21例ICC组织中HBsAg阳性表达率为23.8%(5/21),HBcAg阳性表达率为19.0%(4/21),HBV X 基因检出率为33.3%(7/21)。在31例ECC组织中均未检出HBsAg、HBcAg及HBV X基因。结论 HBV感染与ICC发生关系密切,但可能非ECC发生的危险因素。HBV X基因的整合可能参与肝内胆管上皮细胞的癌变过程。

关键词: 胆管癌, 乙型肝炎病毒, 肝内胆管癌, 肝外胆管癌

Abstract:

Objective To investigate the importance of hepatitis B virus(HBV)infection in the pathogenesis of cholangiocarcinoma(CC)and further clarify the correlation between the occurrence of intrahepatic and extrahepatic cholangiocarcinoma.Methods HBV protein and gene of 52 formalin fixed,paraffin embedded tissues with CC were detected by immunohistochemistry and nest PCR.Results Hepatitis B virus X gene was detectable in 33.3%(7/21)of 21 intrahepatic cholangiocarcinoma cases.Hepatitis B surface antigen(HBsAg)was detectable in 21.7%(5/21)and hepatitis B core antigen(HBcAg)was detectable in 19.0%(4/21)of 21 intrahepatic cholangiocarcinoma cases.In contrast,no HBsAg,HBcAg and hepatitis B virus X gene were detected in any of the 31 extrahepatic cholangiocarcinoma cases.Conclusion HBV infection is a significant risk factor for intrahepatic cholangiocarcinoma,but not for extrahepatic cholangiocarcinoma,in Northeast of China.The integration of HBV gene may be involved in the carcinogenesis of intrahepatic bile duct epithelial cells.

Key words: Cholangiocarcinoma, Hepatitis B virus, Intrahepatic cholangiocarcinoma, Extrahepatic cholangiocarcinoma

中图分类号:

R730.43

吴旖琦, 刘颖, 赵丽, 金晓明. 乙肝病毒蛋白和基因在肝内和肝外胆管癌组织中的检测及意义[J]. 实用肿瘤学杂志, 2017, 31(1): 7-12.

WU Yiqi, LUI Ying, ZHAO Li, JIN Xiaoming. Detection of hepatitis B virus protein and gene in intrahepatic and extrahepatic cholangiocarcinoma tissue[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(1): 7-12.

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乙肝病毒蛋白和基因在肝内和肝外胆管癌组织中的检测及意义

Detection of hepatitis B virus protein and gene in intrahepatic and extrahepatic cholangiocarcinoma tissue

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