番茄红素对荷卵巢癌大鼠免疫功能的影响及其机制研究

doi:10.11904/j.issn.1002-3070.2017.01.003

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (1): 13-17.doi: 10.11904/j.issn.1002-3070.2017.01.003

番茄红素对荷卵巢癌大鼠免疫功能的影响及其机制研究

柴静波¹, 李萍¹, 裴志萍²

1.邯郸市第二医院妇产科(邯郸 056001);
2.邯郸市第二医院外一科

收稿日期:2016-01-27 出版日期:2017-02-28 发布日期:2017-02-20
通讯作者: 柴静波,E-mail:zqzhlx@126.com
作者简介:柴静波,女,(1980-),本科,主治医师,从事妇产科疾病的研究

The effects and mechanism of Lycopene on immune function of ovarian cancer rats

CHAI Jingbo¹, LI Ping¹, PEI Zhiping²

1.Department of Obstetrics,The Second Hospital of Handan,Handan 056001,China;
2.Department of General Surgery,The Second Hospital of Handan

Received:2016-01-27 Online:2017-02-28 Published:2017-02-20

摘要/Abstract

摘要： 目的研究番茄红素(LP)改善荷卵巢癌大鼠免疫功能的作用并探讨其机制。方法将卵巢癌细胞NUTU-19接种于腋窝皮下,制备荷卵巢癌大鼠模型,取60只模型大鼠随机分为模型组、顺铂组(2 mg/kg)和LP(40、20、10 mg/kg)组,每组12只;各治疗组均隔天腹腔注射给药一次,共5次。观察各组大鼠一般生存状态和肿瘤生长状况;称量肿瘤重量并计算抑瘤率,通过HE染色观察肿瘤组织形态结构变化;噻唑蓝(MTT)比色法测定脾淋巴细胞转化率;酶联免疫(ELISA)法测定血清中白细胞介素-2(IL-2)和肿瘤坏死因子-α(TNF-α)含量;流式细胞仪术(Flow cytometry)检测血中T淋巴细胞亚群(CD4⁺、CD8⁺)百分率并计算CD4⁺/CD8⁺。结果与模型组比较,发现LP各组大鼠饮食状况好转,瘤体减小、活动度和硬度较差,肿瘤组织细胞皱缩、呈片状坏死等病变,上述改变以LP 40 mg/kg组最为显著;LP(40、20 mg/kg)组瘤重显著减轻且抑瘤率显著升高(P＜0.05,P＜0.01),脾淋巴细胞转化率显著升高(P＜0.05,P＜0.01),血清中IL-2和TNF-α含量均显著降低(P＜0.05,P＜0.01),血液中CD8⁺百分率显著降低(P＜0.05,P＜0.01),LP 40 mg/kg组CD4⁺百分率显著升高(P＜0.05),LP(40、20 mg/kg)组CD4⁺/CD8⁺显著升高(P＜0.05,P＜0.01)。结论 LP具有提高荷卵巢癌大鼠免疫功能的作用,其机制可能与LP能够有效降低炎症细胞因子(IL-2、TNF-α)含量以及提高CD4⁺/CD8⁺比值有关。

关键词: 番茄红素, 卵巢癌, 大鼠, 免疫功能, 机制

Abstract: Objective To investigate the effects of Lycopene(LP)on immune function of ovarian cancer rats' and its mechanism.Methods Sixty ovarian cancer rat models made by injecting ovarian cancer cell line NUTU-19 were divided into five groups randomly:model group,Cisplatin group(2 mg/kg)and LP(40,20,10 mg/kg)group,n=12;the LP and Cisplatin were given by intraperitoneal injection,once every two days for 5 times.The rats general states and tumor growth conditions were observed,the tumor tissue morphological changes was observed by H&E staining;the tumor weight,inhibition rate,spleen lymphocyte transformation rate were detected;and the content of IL-2 and TNF-α in serum were detected.The present of CD4⁺,CD8⁺ in blood were detected and the ratio of CD4⁺/CD8⁺ was calculated.Results The food situation of the rats in LP groups was improved,tumor was reduced,activity and hardness were poorer,necrosis presented,tumor cell shrinkage and other pathological morphological changes appeared,especially the rats in LP 40 mg/kg group.Compared with model group,the tumor weight in LP(40,20 mg/kg)groups was decreased,the inhibition rate and the spleen lymphocyte transformation rate were increased;the contents of IL-2 and TNF-α were decreased;the CD8⁺ present was decreased,while the CD4⁺ present in LP 40 mg/kg group was increased,the ratio of CD4⁺/CD8⁺ was increased.All of the difference above were significant(P＜0.05,P＜0.01).Conclusion LP can effectively improve the immune function of ovarian cancer rat;which perhaps relates to its effects of reducing the inflammatory cytokines levels and increasing the ratio of CD4⁺/CD8⁺.

Key words: Lycopene, Ovarian cancer, Rat, Immune function, Mechanism

中图分类号:

R737.3

柴静波, 李萍, 裴志萍. 番茄红素对荷卵巢癌大鼠免疫功能的影响及其机制研究[J]. 实用肿瘤学杂志, 2017, 31(1): 13-17.

CHAI Jingbo, LI Ping, PEI Zhiping. The effects and mechanism of Lycopene on immune function of ovarian cancer rats[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(1): 13-17.

参考文献

1 Jemal A,Siegel R,Xu J,et al.Cancer Statistics,2010[J].Ca Cancer Journal for Clinicians,2010,60(5):277-300.
2 Karachaliou N,Pilotto S,Bria E,et al.Platelets and their role in cancer evolution and immune system[J].Transl Lung Cancer Res,2015,4(6):713-720.
3 Codony-Servat J,Rosell R.Cancer stem cells and immunoresistance:clinical implications and solutions[J].Transl Lung Cancer Res,2015,4(6):689-703.
4 De Stefani E,Boffetta P,Oreggia F.Plant foods and risk of laryngeal cancer:a case-control study in Uraguay[J].Int J cancer,2000,87:129-132.
5 Lin HY,Huang BR,Yeh WL,et al.Antineuroinflammatory effects of lycopene via activation of adenosine monophosphate-activated protein kinase-α1/heme oxygenase-1 pathways[J].Neurobiol Aging,2014,35(1):191-202.
6 Aydin S,Tokac M,Taner G,et al.Antioxidant and antigenotoxic effects of lycopene in obstructive jaundice[J].J Surg Res,2013,182(2):285-295.
7 邹党华,谭布珍,黄艳琴,等.小剂量雷公藤内酯醇对荷卵巢癌大鼠体内抑瘤作用的观察[J].江西医学院学报,2009,49(2):110-111.
8 官佳懿,张永红,崔德凤,等.绿原酸对小鼠脾脏淋巴细胞体外增殖和分泌功能的影响[J].中国农学通报,2013,29(29):27-31.
9 陈慰峰.医学免疫学[M].第3版.北京:人民卫生出版社,2001:46-52.
10 Boyman O,Sprent J.The role of interleukin2 during homeostasis and activation of the immune system[J].Nat Rev Immunol,2012,12(3):180-190.
11 Wang Z,Wang G,Zhu X,et al.Interleukin-2 is upregulated in patients with a prolapsed lumbar intervertebral disc and modulates cell proliferation,apoptosis and extracellular matrix metabolism of human nucleus pulposus cells[J].Exp Ther Med,2015,10(6):2437-2443.
12 张建武,闵冬雨,周云,等.番茄红素对H₂O₂致乳鼠心肌细胞氧化应激损伤的保护作用[J].中国实验方剂学杂志,2014,20(12):160-164.
13 Anquetil F,Clavel C,Offer G,et al.IgM and IgA rheumatoid factors purified from rheumatoid arthritis sera boost the Fc receptor-and complement-dependent effector functions of the disease-specific anti-citrullinated protein autoantibodies[J].J Immunol,2015,194(8):3664-3674.
14 Xie J,Qiu ZF,Han Y,et al.The correlation between HLA-DR or CD38 expression in CD8(+)T cells and CD4(+)T cells count in treatment-naive HIV/AIDS patients[J].Zhonghua Nei Ke Za Zhi,2016,55(1):36-39.
15 Liu YL,Wu YB,Zhai K,et al.Determination of interleukin 27-producing CD4 and CD8 T cells for the differentiation between tuberculous and malignant pleural effusions[J].Sci Rep,2016,6:19424-19428.

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番茄红素对荷卵巢癌大鼠免疫功能的影响及其机制研究

The effects and mechanism of Lycopene on immune function of ovarian cancer rats

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