RIZ1蛋白表达与宫颈鳞状细胞癌临床病理参数及放疗敏感性的相关性研究

doi:10.11904/j.issn.1002-3070.2017.05.004

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (5): 405-410.doi: 10.11904/j.issn.1002-3070.2017.05.004

RIZ1蛋白表达与宫颈鳞状细胞癌临床病理参数及放疗敏感性的相关性研究

张云艳, 刘亚琴, 王磊, 张琳, 成海燕, 杨姗姗

哈尔滨医科大学附属肿瘤医院(哈尔滨 150081)

收稿日期:2017-01-18 出版日期:2017-10-28 发布日期:2017-10-24
通讯作者: 杨姗姗,E-mail:doctor_yss@hotmail.com
作者简介:张云艳,女,(1972-),博士,主任医师,从事宫颈癌放疗增敏的相关研究
基金资助:
黑龙江省教育厅课题(12541371)

Relationship between expression of RIZ1 and clinicopathological factors or radiosensitivity in human cervical squamous cell carcinoma

ZHANG Yunyan, LIU Yaqin, WANG Lei, ZHANG Lin, CHENG Haiyan, YANG Shanshan

Harbin Medical University Cancer Hospital,Harbin 150081,China

Received:2017-01-18 Online:2017-10-28 Published:2017-10-24

摘要/Abstract

摘要： 目的探讨宫颈鳞状细胞癌组织中Rb蛋白结合锌指结构基因1(RIZ1)的表达及其与宫颈癌患者临床病理参数及放疗敏感性的关系。方法采用免疫组化法观察RIZ1在159例宫颈鳞癌(Ⅱ b、Ⅲ a 期)患者及20例正常宫颈组织中的表达情况,分析RIZ1在宫颈鳞癌组织中不同组间的差异表达情况,及其与宫颈癌放疗敏感性的相关性。结果与正常宫颈组织相比,RIZ1蛋白在宫颈鳞癌组织中的表达水平明显下降(P＜0.001);RIZ1蛋白的表达水平与宫颈癌的放疗敏感性密切相关(P=0.012),并与FIGO分期(P=0.004)、深肌层浸润(P=0.026)、盆腔淋巴结转移(P=0.021)以及放疗后复发(P=0.005)密切相关,Logistic回归结果提示RIZ1蛋白高表达是宫颈癌放疗敏感性的独立预测因素(P=0.045)。结论 RIZ1可能参与宫颈鳞癌的发生与发展过程,并可能成为评估宫颈鳞癌放疗敏感性的候选标志物。

关键词: 宫颈肿瘤, RIZ1, 免疫组化, 放疗敏感性

Abstract: Objective The aim of this study was to analyze the expression of RIZ1 and its association with clinicopathological factors or radiosensitivity in cervical squamous cell carcinoma.Methods 159 samples from patients with FIGO stage Ⅱ b and Ⅲ a cervical squamous cell carcinoma were collected and preserved before radiotherapy.The expression of RIZ1 in cervical squamous cell carcinoma tissues and 20 normal cervical tissues was assessed by immunohistochemistry.The relationship between the RIZ1 expression and the clinicopathological factors or radiosensitivity in cervical squamous cell carcinoma was analyzed by statistical analysis.Results The expression of RIZ1 protein was down-regulated in patients with cervical cancer(P＜0.001).The level of RIZ1 protein expression was close related to radiosensitivity(P=0.012),FIGO staging(P=0.004),deep myometrial invasion(P=0.026),and pelvic lymph node metastasis(P=0.005).Logistic regression analysis showed that the high expression of RIZ1 protein was an independent predictor of radiosensitivity in cervical cancer(P=0.045).Conclusion RIZ1 may be involved in the occurrence and development process of cervical squamous cell carcinoma and regard as a candidate biomarker for the sensitivity in cervical squamous cell carcinoma in radiotherapy.

Key words: Cervical cancer, RIZ1, Immunohistochemistry, Radiosensitivity

中图分类号:

R735.7

张云艳, 刘亚琴, 王磊, 张琳, 成海燕, 杨姗姗. RIZ1蛋白表达与宫颈鳞状细胞癌临床病理参数及放疗敏感性的相关性研究[J]. 实用肿瘤学杂志, 2017, 31(5): 405-410.

ZHANG Yunyan, LIU Yaqin, WANG Lei, ZHANG Lin, CHENG Haiyan, YANG Shanshan. Relationship between expression of RIZ1 and clinicopathological factors or radiosensitivity in human cervical squamous cell carcinoma[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(5): 405-410.

参考文献

1 Torre LA,Bray F,Siegel RL,et al.Global cancer statistics,2012[J].CA Cancer J Clin,2015,65(2):87-108.
2 Siegel R,Ma J,Zou Z,et al.Cancer statistics,2014[J].CA Cancer J Clin,2014,64(1):9-29.
3 Ashton TM,Fokas E,Kunz-Schughart LA,et al.The anti-malarial atovaquone increases radiosensitivity by alleviating tumour hypoxia[J].Nat Commun,2016,7:12308.
4 Nickson CM,Moori P,Carter RJ,et al.Misregulation of DNA damage repair pathways in HPV-positive head and neck squamous cell carcinoma contributes to cellular radiosensitivity[J].Oncotarget,2017,8(18):29963-29975.
5 Qian X,Tan C,Yang B,et al.Astaxanthin increases radiosensitivity in esophageal squamous cell carcinoma through inducing apoptosis and G2/M arrest[J].Dis Esophagus,2017,30(6):1-7.
6 Lakshmikuttyamma A,Takahashi N,Pastural E,et al.RIZ1 is potential CML tumor suppressor that is down-regulated during disease progression[J].J Hematol Oncol,2009,2:28.
7 Piao GH,Piao WH,He Y,et al.Hyper-methylation of RIZ1 tumor suppressor gene is involved in the early tumorigenesis of hepatocellular carcinoma[J].Histol Histopathol,2008,23(10):1171-1175.
8 Akahira J,Suzuki F,Suzuki T,et al.Decreased expression of RIZ1 and its clinicopathological significance in epithelial ovarian carcinoma:correlation with epigenetic inactivation by aberrant DNA methylation[J].Pathology International,2007,57(11):725-733.
9 Zhang C,Zhu Q,He H,et al.RIZ1:a potential tumor suppressor in glioma[J].BMC Cancer,2015,15:990.
10 Buyse IM,Takahashi E,Huang S,et al.Physical mapping of the retinoblastoma interacting zinc finger gene RIZ to D1S228 on chromosome lp36[J].Genomics,1996,34(1):119-121.
11 Liu L,Shao G,Steele-Perkins G,et al.The retinoblastoma interacting zinc finger gene RIZ produces a PR domain-lacking product through an internal promoter[J].J Biol Chem,1997,272(5):2984-2991.
12 Keun-Cheol K,Geng I,Shi H.Inactivation of a histone methyltransferase by mutations in human cancers[J].Cancer Res,2003,63(22):7619-7623.
13 Canote R,Du Y,Carling T,et al.The tumor suppressor gene RIZ in cancer gene therapy[J].Oncol Rep,2002,9(1):57-60.
14 Dong S,Zhang P,Liang S,et al.The role of the retinoblastoma protein-interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells[J].Oncol Lett,2013,6(6):1656-1662.
15 He L,Yu JX,Liu L,et al.RIZ1,but not the alternative RIZ2 product of the same gene,is underexpressed in breast cancer,and forced RIZ1 expression causes G2-M cell cycle arrest and/or apoptosis[J].Cancer Res,1998,58(19):4238-4244.
16 Jiang G,Liu L,Buyse IM,et al.Decreased RIZ1 expression but not RIZ2 in hepatoma and suppression of hepatoma tumorigenicity by RIZ1[J].Int J Cancer,1999,83(4):541-546.
17 Liu ZY,Wang JY,Liu HH,et al.Retinoblastoma protein-interacting zinc-finger gene 1(RIZ1)dysregulation in human malignant meningiomas[J].Oncogene,2013,32(10):1216-1222.
18 Shadat NM,Koide N,Khuda II,et al.Retinoblastoma protein-interacting zinc finger 1(RIZ1)regulates the proliferation of monocytic leukemia cells via activation of p53[J].Cancer Invest,2010,28(8):806-812.
19 Dong SW,Cui YT,Zhong RR,et al.Decreased expression of retinoblastoma protein-interacting zinc-finger gene 1 in human esophageal squamous cell cancer by DNA methylation[J].Clin Lab,2012,58(1/2):41-51.
20 Jiang GL,Huang S.Adenovirus expressing RIZ1 in tumor suppressor gene therapy of microsatellite-unstable colorectalcancers[J].Cancer Res,2001,61(5):1796-1798.
21 Zhang C,Li H,Wang Y,et al.Epigenetic inactivation of the tumor suppressor gene RIZ1 in hepatocellular carcinoma involves both DNA methylation and histone modifications[J].J Hepatol,2010,53(5):889-895.

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RIZ1蛋白表达与宫颈鳞状细胞癌临床病理参数及放疗敏感性的相关性研究

Relationship between expression of RIZ1 and clinicopathological factors or radiosensitivity in human cervical squamous cell carcinoma

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