抑癌基因PRDM5在HPV16病毒致宫颈鳞癌发生过程中的作用研究

doi:10.11904/j.issn.1002-3070.2018.06.001

实用肿瘤学杂志 ›› 2018, Vol. 32 ›› Issue (6): 483-487.doi: 10.11904/j.issn.1002-3070.2018.06.001

• 基础研究 • 下一篇

抑癌基因PRDM5在HPV16病毒致宫颈鳞癌发生过程中的作用研究

成海燕, 马晶, 冯紫微, 黄可金, 王玉霞, 张云艳

哈尔滨医科大学附属肿瘤医院(哈尔滨 150081)

收稿日期:2018-05-29 出版日期:2018-12-28 发布日期:2018-12-27
通讯作者: 张云艳, E-mail:zhangyunyan_1972@163.com
作者简介:成海燕, 女, (1979-), 博士, 副主任医师, 从事宫颈癌基因诊断和治疗的研究。
基金资助:
国家自然科学基金(编号:8167102096);黑龙江省青年科学基金项目(编号:QC2014C105);黑龙江省卫生厅课题(编号:2011-129);黑龙江省肿瘤医院院内科研启动资金(编号:JJ2010-22)

Study on the mediating role of tumor suppressor gene PRDM5 in cervical squamous cell carcinoma induced by HPV16 virus

CHENG Haiyan, MA Jing, FENG Ziwei, HUANG Kejin, WANG Yuxia, ZHANG Yunyan

Harbin Medical University Cancer Hospital, Harbin 150081, China

Received:2018-05-29 Online:2018-12-28 Published:2018-12-27

摘要/Abstract

摘要： 目的探讨抑癌基因PRDM5表达下降是否是HPV16病毒感染导致宫颈癌发生的机制之一。方法采用免疫组化法检测宫颈癌组织及正常宫颈组织中PRDM5蛋白及HPV16 E6/HPV18 E6蛋白的表达;HPV16 E6 shRNA质粒转染SiHa细胞后, 应用RT-PCR及 Western blot法检测PRDM5的表达。结果 HPV16/18 E6在宫颈癌组织中表达阳性率明显高于正常宫颈组织;宫颈癌组织中PRDM5蛋白表达水平较正常宫颈组织中表达水平低;将HPV16 E6 shRNA3转染SiHa细胞干扰HPV16 E6表达后, 发现SiHa细胞中PRDM5 mRNA及蛋白表达上调。结论 PRDM5可能介导HPV16病毒感染导致宫颈癌的发生发展过程。

关键词: 宫颈癌, PRDM5, HPV16, shRNA

Abstract: Objective The aim of this study was to explore whether the down-regulation of tumor suppressor gene PRDM5 is one of the mechanisms of HPV16 virus infection leading to cervical cancer. Methods The expressions of PRDM5 protein and HPV16 E6/HPV18 E6 protein in cervical cancer tissues and normal cervical tissues were detected by immunohistochemistry.After transfected with HPV16 E6 shRNA plasmid, the expression of PRDM5 gene was detected in SiHa cells by RT-PCR and Western blot. Results The positive expression rate of HPV 16/18 E6 in cervical cancer tissues was significantly higher than that in normal cervical tissues.The expression level of PRDM5 protein in cervical cancer tissues was lower than that in normal cervical tissues.After HPV16 E6 shRNA3 was transfected into SiHa cells to interfere with the expression of HPV16 E6 gene, the expression of PRDM5 at mRNA and protein levels was up-regulated in SiHa cells. Conclusion PRDM5 may mediate the development of cervical cancer caused by HPV16 virus infection.

Key words: Cervical cancer, PRDM5, HPV16, shRNA

中图分类号:

R735.7

成海燕, 马晶, 冯紫微, 黄可金, 王玉霞, 张云艳. 抑癌基因PRDM5在HPV16病毒致宫颈鳞癌发生过程中的作用研究[J]. 实用肿瘤学杂志, 2018, 32(6): 483-487.

CHENG Haiyan, MA Jing, FENG Ziwei, HUANG Kejin, WANG Yuxia, ZHANG Yunyan. Study on the mediating role of tumor suppressor gene PRDM5 in cervical squamous cell carcinoma induced by HPV16 virus[J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(6): 483-487.

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抑癌基因PRDM5在HPV16病毒致宫颈鳞癌发生过程中的作用研究

Study on the mediating role of tumor suppressor gene PRDM5 in cervical squamous cell carcinoma induced by HPV16 virus

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