红松松塔提取物多聚苯丙素-多糖复合物诱导人乳腺癌MCF-7细胞凋亡

doi:10.11904/j.issn.1002-3070.2019.04.001

实用肿瘤学杂志 ›› 2019, Vol. 33 ›› Issue (4): 289-293.doi: 10.11904/j.issn.1002-3070.2019.04.001

• 基础研究 • 下一篇

红松松塔提取物多聚苯丙素-多糖复合物诱导人乳腺癌MCF-7细胞凋亡

安巍巍, 唐雅莉, 赵莲花, 杨悦, 王新玲

哈尔滨医科大学肿瘤防治研究所(哈尔滨 150081)

收稿日期:2018-10-10 发布日期:2019-08-30
通讯作者: 安巍巍,E-mail:1103514848@qq.com
作者简介:安巍巍,女,(1975-),博士,副研究员,从事肿瘤药理学的研究。
基金资助:
黑龙江省教育厅科学技术研究(编号:12521199);哈尔滨市科学技术局科技创新人才项目(编号:2015RAQYJ065)

Polyphenylene-polysaccharide complex from pinus koraiensis extract induces apoptosis in human breast cancer MCF-7 cells

AN Weiwei, TANG Yali, ZHAO Lianhua, YANG Yue, WANG Xinling

Institute of Cancer Prevention and Treatment,Harbin Medical University,Harbin 150081,China

Received:2018-10-10 Published:2019-08-30

摘要/Abstract

摘要： 目的研究红松松塔提取物多聚苯丙素-多糖复合物(Polyphenylpropenoid-polysaccharide complex,PPC)诱导人乳腺癌MCF-7细胞凋亡的机制。方法用体外培养的MCF-7细胞与不同浓度PPC作用不同时间,应用MTT法、荧光染色法、凋亡检测试剂盒、DNA凝胶电泳及Western blot检测PPC对MCF-7细胞增殖的影响和诱导凋亡的作用。结果 PPC可诱导MCF-7细胞发生凋亡。细胞凋亡时Caspase-3和Caspase-9的酶活力升高,Caspase-3前体酶蛋白表达下降;Caspase-3抑制剂(z-DEVE-fmk)可部分抑制PPC诱导的细胞凋亡,并抑制Caspase-3前体酶的活化。结论 PPC通过激活Caspase家族诱导MCF-7细胞凋亡。

关键词: 红松松塔, 乳腺癌, MCF-7细胞, 细胞凋亡, Caspase-3, Caspase-3抑制剂

Abstract: Objective The mechanism of apoptosis induced by polyphenyl propenoid-polysaccharide complex(PPC)in human breast cancer MCF-7 cells was studied.Methods MCF-7 cells were cultured with different concentrations of PPC for different time.The effects of PPC on proliferation and apoptosis were detected in MCF-7 cells by MTT assay,fluorescent staining,apoptosis detection kit,DNA gel electrophoresis and Western blot.Results PPC induced apoptosis in MCF-7 cells.When apoptosis occurred,the enzyme activities of caspase-3 and-9 were increased,and the expression of pro-caspase-3 protein was decreased.Caspase-3 inhibitor(z-DEVE-fmk)could partially inhibit PPC-induced apoptosis and inhibit activation of caspase-3 precursor enzyme.Conclusion PPC induces apoptosis of MCF-7 cells by activating caspase family.

Key words: Pine Cone, Breast cancer, MCF-7 cells, Apoptosis, Caspase-3, Caspase-3 inhibitor

中图分类号:

R961
R73.36

安巍巍, 唐雅莉, 赵莲花, 杨悦, 王新玲. 红松松塔提取物多聚苯丙素-多糖复合物诱导人乳腺癌MCF-7细胞凋亡[J]. 实用肿瘤学杂志, 2019, 33(4): 289-293.

AN Weiwei, TANG Yali, ZHAO Lianhua, YANG Yue, WANG Xinling. Polyphenylene-polysaccharide complex from pinus koraiensis extract induces apoptosis in human breast cancer MCF-7 cells[J]. Journal of Practical Oncology, 2019, 33(4): 289-293.

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红松松塔提取物多聚苯丙素-多糖复合物诱导人乳腺癌MCF-7细胞凋亡

Polyphenylene-polysaccharide complex from pinus koraiensis extract induces apoptosis in human breast cancer MCF-7 cells

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