血根碱通过诱导细胞凋亡抑制卵巢癌肿瘤生长的机制研究

doi:10.11904/j.issn.1002-3070.2019.04.004

摘要/Abstract

摘要： 目的探讨血根碱对卵巢癌细胞SKOV3生长抑制的作用机制。方法采用CCK-8方法检测血根碱处理后卵巢癌细胞SKOV3的生长情况;采用流式细胞仪检测血根碱对细胞凋亡的影响;采用分光光度计法检测血根碱对活性氧产生的影响;采用小鼠卵巢癌肿瘤模型检测血根碱对小鼠肿瘤生长的抑制作用。结果血根碱可以促进卵巢癌细胞SKOV3发生凋亡,并且呈剂量和时间依赖性。血根碱诱导的细胞凋亡与活性氧的产生相关,并激活了c-Jun-N-末端激酶(JNK)和核因子-κB(NF-κB)信号通路。在卵巢癌异种移植小鼠模型中,用血根碱静脉注射小鼠后,与对照组相比,血根碱处理组导致小鼠肿瘤生长明显迟缓。体外TUNEL染色表明血根碱显著诱导肿瘤组织凋亡。结论血根碱可以显著抑制卵巢癌细胞SKOV3的生长,诱导细胞凋亡,增加活性氧的产生,抑制卵巢癌肿瘤生长。

关键词: 血根碱, 凋亡, 活性氧, 卵巢癌

Abstract: Objective The aim of this study was to investigate the mechanism of sanguinarine(SANG)on the inhibitory proliferation in ovarian cancer SKOV3 cells.Methods CCK-8 assay was used to detect proliferation of SKOV3 cells.Flow cytometry was used to detect the effect of SANG on apoptosis in SKOV3 cells.The spectrophotometer was used to detect the production of reactive oxygen species(ROS)by SANG.The mouse ovarian cancer xenograft model was used to detect the inhibitory effect of SANG on tumor growth.Results SANG promoted apoptosis in SKOV3 cells in a dose-and time-dependent manner.The SANG-induced apoptosis was associated with the production of ROS,Activated the c-Jun-N-terminal kinase(JNK)and nuclear factor-κB(NF-κB)signaling pathways.In mouse model of ovarian cancer xenografts,after intravenous injection of mice with SANG,SANG was significantly inhibited the growth of ovarian cancer xenografts when compared to the control group.SANG also significantly induced apoptosis in ovarian cancer xenografts.Conclusion SANG can significantly inhibit the proliferation of ovarian cancer SKOV3 cells,induce apoptosis,increase the production of ROS,and inhibit the growth of ovarian cancer.

Key words: Sanguinarine, Apoptosis, Reactive oxygen species, Ovarian cancer

中图分类号:

R737.3

张蓓蕾, 李怡, 吴涛, 姜锋, 赵宏喜, 李艳红. 血根碱通过诱导细胞凋亡抑制卵巢癌肿瘤生长的机制研究[J]. 实用肿瘤学杂志, 2019, 33(4): 305-309.

ZHANG Beilei, LI Yi, WU Tao, JIANG Feng, ZHAO Hongxi, LI Yanhong. Mechanism study of sanguinarine inhibiting tumor growth of ovarian cancer by apoptosis induction[J]. Journal of Practical Oncology, 2019, 33(4): 305-309.

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