ABCG2及CD133在体内外不同分化胃癌中的表达水平研究

doi:10.3969/j.issn.1002-3070.2012.04.008

实用肿瘤学杂志 ›› 2012, Vol. 26 ›› Issue (4): 319-323.doi: 10.3969/j.issn.1002-3070.2012.04.008

ABCG2及CD133在体内外不同分化胃癌中的表达水平研究

李惠, 刘冰熔

哈尔滨医科大学附属第二医院消化内科(哈尔滨 150086)

收稿日期:2012-03-08 出版日期:2012-08-28 发布日期:2014-12-03
通讯作者: 刘冰熔,E-mail:melanielihui@hotmail.com
作者简介:李惠,女,(1982-),硕士,主治医师,从事IBD及胃肠道肿瘤发生发展的免疫学机制
基金资助:
哈尔滨医科大学附属第二医院青年基金资助(QN2008-13)

Expression of ABCG2 and CD133 in varied differentiated gastric cancer in vivo and in vitro

LI Hui,LIU Bingrong

Department of Gastroenterology and Hepatology,The Second Affiliated Hospital of Harbin Medical University,Harbin 150086

Received:2012-03-08 Online:2012-08-28 Published:2014-12-03

摘要/Abstract

摘要： 目的研究ABCG2及CD133两种待定肿瘤干细胞标志物的表达与不同分化胃腺癌的数量关系。方法 (1)在78例人胃腺癌组织中以免疫荧光法检测ABCG2和CD133的表达;(2)以流式细胞技术对未分化胃癌细胞系HGC-27、低分化胃癌细胞系BGC-823及中-低分化胃腺癌细胞系SGC-7901中ABCG2、CD133的表达进行测定;(3)用上述三种细胞系建立裸鼠胃壁移植成瘤模型,采用免疫荧光染色的方法对体内环境下ABCG2和CD133在移植瘤中的表达情况进行检测。结果 (1)ABCG2和CD133的表达均与肿瘤分化程度相关。在人胃腺癌标本中,两种标志物的表达随着胃癌恶性程度的增加而增加,分化越差的胃癌ABCG2、CD133表达水平越高;并且根据Lauren分型,CD133在人弥散型胃癌中的表达高于肠型胃癌;(2)未分化细胞系HGC-27中ABCG2和CD133的表达要比中-低分化细胞系高;(3)三种胃癌细胞移植鼠模型中移植瘤组织ABCG2和CD133表达水平与人胃癌组织和不同分化胃癌细胞系的表达水平相似,低分化胃癌细胞株HGC-27移植瘤ABCG2、CD133表达水平最高。结论肿瘤干细胞标志物ABCG2和CD133在不同分化胃癌中的表达有差异,且低分化胃癌其肿瘤干细胞标志物表达水平较高。

Abstract: Objective To determine whether quantitative relationship exists between the expressions of 2 cancer stem cell markers,ABCG2 and CD133,and differentiation degrees of gastric cancer.Methods (1)The expressions of ABCG2 and CD133 were detected in 78 cases of human gastric adenocarcinomas by immunoflurescence assay;(2)2 markers were also detected in 3 gastric cancer cell lines,undifferentiated gastric cancer cell line HGC-27,poorly differentiated gastric cancer cell line BGC-823,and moderately-poorly differentiated gastric adenocarcinoma cell line SGC-7901,in vitro by flow cytometric analysis.(3)Gastric xenotransplant cancers in nude mice were constructed to compare the malignancy of 3 separated cell lines.Expressions of 2 markers were detected in vivo by immunoflurescent staining.Results (1)Expressions of 2 markers were generally correlated with the differentiations of gastric cancers.In human gastric adenocarcinoma,the expressions of 2 markers were increased with the elevation of the malignancy grades of gastric cancers.In addition,the expressions of CD133 were higher in diffuse type than those in intestinal type of human gastric cancers.(2)Undifferentiated cell line HGC-27 expressed more markers than moderately-poorly differentiated cell line SGC-7901.(3)The similar result had been observed in xenotransplant tumors which arouse from 3 cell lines.Conclusion The expressions of cancer stem cell markers ABCG2 and CD133 differed in various differentiation degrees of gastric cancers,poorly differentiated gastric cancer expressed relatively more.

中图分类号:

R735.2

李惠, 刘冰熔. ABCG2及CD133在体内外不同分化胃癌中的表达水平研究[J]. 实用肿瘤学杂志, 2012, 26(4): 319-323.

LI Hui,LIU Bingrong. Expression of ABCG2 and CD133 in varied differentiated gastric cancer in vivo and in vitro[J]. PRACTICAL ONCOLOGY JOURNAL, 2012, 26(4): 319-323.

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ABCG2及CD133在体内外不同分化胃癌中的表达水平研究

Expression of ABCG2 and CD133 in varied differentiated gastric cancer in vivo and in vitro

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