Abstract: Objective To investigate the effect of Survivin gene on the growth of human colorectal cancer HT-29 cells and to explore the feasibility of gene therapy in human colorectal carcinoma.Methods The human colorectal cancer HT-29 cell line was treated with ASODNs.The effects of Survivin-ASODN on the growth of HT-29 were studied by cell colony assay, reverse transcription polymerse chain reaction.The invasive ability was measured by number of cells to penetrate polycarbonates coated with matrigel.Results The number of HT-29 cells colony was inhibited after the cells were treated for 48 hours.The cell proliferation was inhibited effectively, too.The expression of Survivin mRNA decreased sharply in HT-29 cells treated with ASODNs.The cells number that penetrated polycarbonates was significantly lower in the ASODNs group than that of control group.Conclusions The antisense oligodeoxynucleotides were capable of inhibiting the proliferation and the invasive ability of human colorectal carcinoma HT-29 cells.