肝细胞移植治疗大鼠急性肝衰竭的疗效观察

doi:10.3969/j.issn.1002-3070.2009.06.006

实用肿瘤学杂志 ›› 2009, Vol. 23 ›› Issue (6): 520-523.doi: 10.3969/j.issn.1002-3070.2009.06.006

肝细胞移植治疗大鼠急性肝衰竭的疗效观察

孙大为¹, 张松岩¹, 郝军², 李海洋³

1.哈尔滨医科大学附属肿瘤医院腹外科(哈尔滨 150081);
2.黑龙江省卫生厅疾控处;
3.贵阳医学院附属医院

收稿日期:2009-08-26 出版日期:2009-12-25 发布日期:2012-02-21
作者简介:孙大为, 男, (1981-), 住院医师, 硕士, 从事腹部肿瘤外科研究

Study on effect of hepatocytes transplantation in rats with acute liver failure

SUN Dawei¹, ZHANG Songyan¹, HAO Jun², LI Haiyang³

1.Department of Abdominal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin 150081;
2.Health Department of Heilongjiang Province;
3.Department of Hepatobiliary Surgery, Affiliated Hospital of Guiyang Medical College, Guiyang 550004

Received:2009-08-26 Online:2009-12-25 Published:2012-02-21

摘要/Abstract

摘要： 目的观察肝细胞移植治疗大鼠急性肝衰竭的疗效, 探讨原代肝细胞提取方法, 观察体外培养的大鼠肝细胞生存情况。方法利用胶原酶消化法分离大鼠肝细胞, 用D-氨基半乳糖(D-GalactosamineN, D-GalN)制作大鼠急性肝功能衰竭模型, 并以此模型为基础行大鼠同种异体肝细胞移植实验。结果分离的肝脏实质细胞(Hepatocyte)的存活率在87%-95%。平均每只大鼠肝脏可分离出7.8×10⁷个肝脏实质细胞。肝细胞移植组大鼠7天的存活率为54.2%(13/24), 对照组8.3%(2/24), 移植组大鼠存活率与非移植组大鼠存活率之间具有显著性差异(P＜0.005)。结论经腹腔移植同种异体肝细胞可明显改善D-GalN诱导的急性肝衰竭大鼠的存活率, 腹腔肝细胞移植是治疗急性肝衰竭安全有效的方法。

Abstract: Objective To study the effect of hepatocyte transplantation(HCT)intraperitoneal in rats with acute liver failure(ALF)which were induced by D-galactosamineN, and to discuss whether this is an effective way.Methods ALF animals were induced by D-GalN.HCT was received 48h after intoxication.GroupⅠreceived 2.5×10⁷hepatocytes intraperitoneally.GroupⅡreceived 2.5mL NS intraperitoneally.The survival rates, liver function, liver histology and biologic characteristics of transplanted cells were assessed.Survival rate of groupⅠwas much higher than that of groupⅡ(54.2% vs 8.3%, P＜0.005), the liver function and liver histology were partially improved in groupⅠas compared with groupⅡ.Conclusion Survival rate of ALF rats intoxicated with D-GalN could be improved, the liver function and liver histology could also be partially improved, via intraperitoneal HCT.

中图分类号:

孙大为, 张松岩, 郝军, 李海洋. 肝细胞移植治疗大鼠急性肝衰竭的疗效观察[J]. 实用肿瘤学杂志, 2009, 23(6): 520-523.

SUN Dawei, ZHANG Songyan, HAO Jun, LI Haiyang. Study on effect of hepatocytes transplantation in rats with acute liver failure[J]. PRACTICAL ONCOLOGY JOURNAL, 2009, 23(6): 520-523.

肝细胞移植治疗大鼠急性肝衰竭的疗效观察

Study on effect of hepatocytes transplantation in rats with acute liver failure

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