Abstract: Objective To investigate the relationship between the immunohistochemistry expression of p53,Ki-67,TopoⅡ,GST,P170 in triple-negative breast cancer(TNBC)and its prognostic significance.Methods The data of 515 in our hospital were retrospectively analyzed.According to the expression of ER、PR、HER-2,we categarized 515 invasive breast cancer into 3 Groups:HR(ER+/PR + HER-2-),triple negative(ER-,PR-,HER2-),and HER+2(ER-,PR-,HER2+).The expression of p53,Ki-67,TopoⅡ,GST,P170 was detected by immunohistochemical technology in 88 cases of TNBC and their prognostic significance were compared with that of the other two subtypes of non-TNBC.The clinicopathological features,disease free survival rate and overall survival rate of these three subtype were analyzed.Results The expression rate of p53,P170,Ki-67,GSTin TNBC(59.5%、78.6%、19.0%、66.7%)is higher than that of non-TNBC.Among 5-year disease free survival in TNBC,the recurrence rate and death rate is 23.9% and 22.7%,and triple-negative breast cancer has an increased likelihood of distant recurrence(hazard ratio,2.46;confidence interval,1.04-5.84;P＜0.05)and death(hazard ratio,2.77;confidence interval,1.09-7.02;P＜0.05).Conclusions High expression of p53,Ki-67,TopoⅡ,GST,P170 were correlated with the poor prognostic of TNBC.