AICARFT及mTOR在人肺腺癌亲本株A549及培美曲塞耐药株A549/PEM的表达及意义

doi:10.11904/j.issn.1002-3070.2015.04.010

Abstract

Abstract: Objective To investigate the expression of AICARFT and mTOR in lung adenocarcinoma cell line A549 and pemetrexed resistant human lung adenocarcinoma cell line A549/PEM.Methods The distribution of cell cycle was evaluated by flow cytometry assay.The expression of AICARFT and mTOR in A549 and A549/PEM were detected by RT-PCR and Western blot.Results Flow cytometry analysis showed that S phase in A549/PEM was higher than that in A549((36.61±1.36)% vs(27.16±1.08)%(P=0.001)).mRNA over expression of AICARFT and mTOR in A549/PEM were detected by RT-PCR(P＜0.05).Protein over expression of AICARFT and mTOR in A549/PEM were detected by Western blot.(P＜0.05).Conclusion High level of AICARFT and mTOR may be connected with pemetrexed acquired drug resistance in lung adenocarcinoma.The cycle of pemetrexed resistant human lung adenocarcinoma cancer cell line A549/PEM is redistributed.

Key words: AICARFT, mTOR, Resistant, A549, A549/PEM

CLC Number:

R734.2

ZHANG Dan,WANG Hongyang,HUANG Yan,WANG Limin. The expression and significance of AICARFT and mTOR in pemetrexed resistant human lung adenocarcinoma cancer cell line A549/PEM[J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(4): 337-341.

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The expression and significance of AICARFT and mTOR in pemetrexed resistant human lung adenocarcinoma cancer cell line A549/PEM

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