脑胶质瘤中RAD18的表达与放射治疗抵抗相关性分析

doi:10.11904/j.issn.1002-3070.2017.04.001

Abstract

Abstract: Objective The objective of this study was to investigate the relationship between the expression of RAD18 and radiation resistance in glioblastoma multiforme(GBM)and to provide a new therapeutic target for improving the radiation resistance of GBM.Methods Human glioma A172 cells were transfected into blank and RAD18-containing plasmid vector.The cell proliferation of two groups after the same dose radiation was detected by cloning assay.The mRNA expression of RAD18 in primary and recurrent GBM samples after close proximity treatment were detected by qRT-PCR.All data were analyzed statistically.Results The proliferation of GBM cells transfected with RAD18 plasmid was higher than that of cells transfected with blank plasmid after radiation therapy(P＜0.001).The expression level of RAD18 mRNA in recurrent GBM was higher than that in the untreated radioactive granules primary GBM(P＜0.01).Conclusion The resistance of recurrent GBM to radiotherapy may be associated with the overexpression of RAD18 protein.

Key words: Glioblastoma multiforme, RAD18, Radioactive particles

CLC Number:

R739.41

XU Yanbin,MA Xudong,XIE Chen,ZHAN Qi,REN Fubin. Correlation between RAD18 expression and radiotherapy resistance in glioblastoma multiforme[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(4): 289-293.

References

1 Opyrchal M,Allen C,Iankov I,et al.Effective radiovirotherapy for malignant gliomas by using oncolytic measles virus strains encoding the sodium iodide symporter(MV-NIS)[J].Hum Gene Ther,2012,23(4):419-427.
2 徐延斌,李建华.放射性粒子治疗与基因治疗神经胶质瘤的概况[J].实用肿瘤学杂志,2015,29(2):153-156.
3 Wang W,Long L,Wang L,et al.Knockdown of Cathepsin L promotes radiosensitivity of glioma stem cells both in vivo and in vitro[J].Cancer Lett,2016,371(2):274-284.
4 Yang Y,Durando M,Smith-Roe SL,et al.Cell cycle stage-specific roles of Rad18 in tolerance and repair of oxidative DNA damage[J].Nucleic Acids Res,2013,41(4):2296-2312.
5 Franceschi S,Tomei S,Mazzanti CM,et al.Association between RAD51 rs1801320 and susceptibility to glioblastoma[J].J Neurooncol,2016,126(2):265-270.
6 Masuda Y,Suzuki M,Kawai H,et al.Asymmetric nature of two subunits of rad18,a ring-type ubiquitin ligase E3,in the human rad6a-rad18 ternary complex[J].Nucleic Acids Res,2012,40(3):1065-1076.
7 Liu RL,Dong Y,Deng YZ,et al.Tumor suppressor miR-145 reverses drug resistance by directly targeting DNA damage-related gene RAD18 in colorectal cancer[J].Tumour Biol,2015,36(7):5011-5019.
8 Wong RP,Aguissa-Toure AH,Wani AA,et al.Elevated expression of rad18 regulates melanoma cell proliferation[J].Pigment Cell Melanoma Res,2012,25(2):213-218.
9 Xie C,Wang H,Cheng H,et al.RAD18 mediates resistance to ionizing radiation in human glioma cells[J].Biochem Biophys Res Commun,2014,445(1):263-268.
10 Durando M,Tateishi S,Vaziri C.A non-catalytic role of DNA polymerase eta in recruiting Rad18 and promoting PCNA monoubiquitination at stalled replication forks[J].Nucleic Acids Res,2013,41(5):3079-3093.
11 Hibbert RG,Huang A,Boelens R,et al.E3 ligase rad18 promotes monoubiquitination rather than ubiquitin chain formation by E2 enzyme rad6[J].Proc Natl Acad Sci U S A,2011,108(14):5590-5595.
12 Huttner D,Ulrich HD.Cooperation of replication protein A with the ubiquitin ligase Rad18 in DNA damage bypass[J].Cell Cycle,2008,7(23):3629-3633.
13 Bachl J,Ertongur I,Jungnickel B.Involvement of Rad18 in somatic hypermutation[J].Proc Natl Acad Sci U S A,2006,103(32):12081-12086.
14 Palle K,Vaziri C.Rad18 E3 ubiquitin ligase activity mediates fanconi anemia pathway activation and cell survival following DNA topoisomerase 1 inhibition[J].Cell Cycle,2011,10(10):1625-1638.
15 顾昕.自噬在人肺腺癌细胞放疗抵抗形成中的作用及机制研究[D].上海:第二军医大学,2015:1-69.

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Correlation between RAD18 expression and radiotherapy resistance in glioblastoma multiforme

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