MicroRNA-150对上皮性卵巢癌细胞增殖、凋亡和侵袭转移能力的影响

doi:10.11904/j.issn.1002-3070.2018.03.003

Abstract

Abstract: Objective The aim of this study was to investigate the expression of microRNA-150(miR-150)in human epithelial ovarian cancer cells and its effect on proliferation,apoptosis,invasion and metastasis of human epithelial ovarian cancer cells.Methods The expression level of miR-150 in cells from each treatment group was detected by Real-Time PCR(qRT-PCR);effects of proliferation,apoptosis,invasion and metastasis of epithelial ovarian cancer cells was investigated by MTT,flow cytometry,and transwell assays.Results Compared with normal ovarian epithelial cells(T29),the expression of miR-150 was significantly decreased in epithelial ovarian cancer cells(A2780 and OVCAR3)(P＜0.01); After transfection miR-150 mimic,the expression of miR-150 in A2780 and OVCAR3 cells was significantly increased(P＜0.01);After 3 d of transfection,the OD values of the miR-150 mimic group(A2780:1.12±0.03;OVCAR3:1.91±0.03)were lower than that in the blank group(A2780:2.35±0.09;OVCAR3:2.63±0.07)and the miR-150 NC group(A2780:2.18±0.07;OVCAR3:2.43±0.11)(P＜0.01);The apoptotic rate in the miR-150 mimic group(A2780:16.10±0.58%;OVCAR3:15.16±1.30%) were significantly increased when compared to the blank group(A2780:10.07±0.66%;OVCAR3:3.81±0.24%) and the miR-150 NC group(A2780:10.36±1.08%;OVCAR3:4.89±0.07%)(P＜0.01);The number of transmembrane cells in the miR-150 mimic group(A2780:38.67±2.03;OVCAR3:28.67±2.03)was higher than that in the blank group(A2780:76.30±7.45;OVCAR3:55.67±3.18)and the miR-150 NC group(A2780:74.33±5.78;OVCAR3:56.33±3.84)(P＜0.01).Conclusion The decreased expression of miR-150 in epithelial cancer cells may be one of the mechanisms of proliferation,invasion and metastasis of epithelial ovarian cancer.Up-regulation of miR-150 may inhibit the proliferation of epithelial ovarian cancer cells and promote apoptosis to reduce the abilities of invasion and metastasis in epithelial ovarian cancer cells.

Key words: Epithelial ovarian cancer, miR-150, Proliferation, Apoptosis, Invasion

CLC Number:

R737.31

LI Wenhui, LV Bowen, QIAN Jun, SU Liju, YANG Tongshu, QIAN Jingrong, WANG Jie. Effect of microRNA-150 on proliferation,apoptosis,invasion and metastasis of epithelial ovarian cancer cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(3): 208-213.

References

1 Siegel R,NaishadhamD.Cancer statistics,2013[J].CA:A Cancer Journal for Clinicians,2013,63(1):11-30.
2 Allemani C,Matsuda T,Di Carlo V,et al.Global surveillance of trends in cancer survival 2000-14(CONCORD-3):analysis of individual records for 37 513 025 patients diagnosed with one of 18 cancers from 322 population-based registries in 71 countries[J]Lancet,2018,391(10125):1023-1075.
3 Romero I.Minirebiew:human ovarian cancer:biology,current management,and paths to personalizing therapy[J].Endocrinology,2012,153(4):1593-1602.
4 Suh DH,Kim JW,Kim K,et al.Major clinical research advances in gynecologic cancer in 2012[J].J Gynecol Oncol,2013,24(1):66-82.
5 Zhou Y,Wang M,Wu J,et al.The clinicopathological significance of miR-1307 in chemotherapy resistant epithelial ovarian cancer[J].J Ovarian Res,2015,8:23.
6 Pasquinelli AE.MicroRNAs and their targets:recognition,regulation and an emerging reciprocal relationship[J].Nat Rev Genet,2012,13(4):271-282.
7 Alvarez-Garcia I,Miska EA.MicroRNA functions in animal development and human disease[J].Development,2005,132(21):4653-4662.
8 Xu L,Li H,Su L,et al.MicroRNA-455 inhibits cell proliferation and invasion of epithelial ovarian cancer by directly targeting Notch1[J].Mol Med Rep,2017,16(6):9777-9785.
9 Yang A,Wang X,Yu C,et al.microRNA-494 is a potential prognostic marker and inhibits cellular proliferation,migration and invasion by targeting SIRT1 in epithelial ovarian cancer[J].Oncol Lett,2017,14(3):3177-3184.
10 Guan R,Cai S,Sun M.Upregulation of miR-520b promotes ovarian cancer growth.[J].Oncol Lett,2017,14(3):3155-3161.
11 Wu RL,Ali S,Bandyopadhyay S,et al.Comparative analysis of differentially expressed miRNA and their downstream mRNAs in ovarian cancer and its associated endometriosis[J].J Cancer Sci Ther,2015,7(7):258-265.
12 Zhang S,Zhang JY,Lu LJ,et al.MiR-630 promotes epithelial ovarian cancer proliferation and invasion via targeting KLF6[J].Eur Rev Med Pharmacol Sci,2017,21(20):4542-4547.
13 Hou M,Zuo X,Li C,et al.MiR-29b regulates oxidative stress by targeting SIRT1 in ovarian cancer cells[J].Cell Physiol Biochem,2017,43(5):1767-1776.
14 Bai D,Sun H,Wang X,et al.MiR-150 inhibits cell growth in vitro and in vivo by restraining the RAB11A/WNT/β-Catenin pathway in thyroid cancer.[J].Med Sci Monit,2017,23:4885-4894.
15 Wang W H,Chen J,Zhao F,et al.MiR-150-5p suppresses colorectal cancer cell migration and invasion through targeting MUC4.[J].Asian Pac J Cancer Prev,2014,15(15):6369-6273.
16 Sun W,Zhang Z,Wang J,et al.MicroRNA-150 supresses cell proliferation and metastasis in hepaocellular carcinoma by inhibiting the GAB1-ERX axis.[J].Oncotarget,2016,7(10):11595-11608.
17 Cao M,Hou D,Liang H,et al.miR-150 promotes the proliferation and migration of lung cancer cells by targeting SRC kinase signalling inhibitor 1.[J].Eur J Cancer,2014,50(5):1013-1024.
18 Li J,Hu L,Tian C,at al.microRNA-150 promotes cervical cancer cell growth and survival by targeting FOXO4[J].BMC Mol Biol,2015,16:24.
19 Wuerkenbieke D,Wang J,Li Y.miRNA-150 downregulation promotes pertuzumab resistance in ovarian cancer cells via AKT actination[J].Arch Gynecol Obstet,2015,292(5):1109-1116.

[1]	QIN Wen,ZHAO Nannan,HAN Yonghuan,XU Yajun. Expression of ZNF436 in gastric cancer and its effect on the migration and invasion of gastric cancer cells by WNT/β-catenin [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(3): 222-227.
[2]	LIU Zhiqiang,CHU Yanjie,LIU Jing,WANG Yanbo. Atractylenolide I regulates proliferation and invasion of lung cancer A549 cells through the TLR4/MyD88 pathway [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(3): 228-232.
[3]	LV Bowen, QIAN Jun, WANG Jie, QIAN Jingrong, LU Yingying, SU Liju, YANG Tongshu, LI Wenhui. Expression of MiR-455-5p in epithelial ovarian cancer and its target gene function [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(2): 115-121.
[4]	BAI Xiaobin, HUO Longwei, XIE Wanfu, XU Gaofeng, WANG Maode. Expression of Chk1 in glioblastoma and its association with tumor biological behavior and prognosis survival [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(2): 122-127.
[5]	YUAN Guixia, LI Yan, FENG Wanqi, XIA Xiaojuan, LI Xu. Mechanism of tumor suppressor gene DKK2 inhibiting proliferation of nephroblastoma cells in children [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(1): 14-20.
[6]	SONG Ling, FU Qiong. Study of the effect of astragalin on proliferation of ovarian cancer cells by inhibiting the glycolytic pathway induced Via HIF-1α [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(6): 503-509.
[7]	LIU Juan, ZHANG Shutian. Advances in research on the mechanism of cyclin-dependent kinase inhibitor 3(CDKN3)in tumors [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(6): 575-578.
[8]	LI Wanzhen, YIN Jun, LI Ping, LIN Xiaohui, ZHANG Guoqian, ZHANG Shuxu. The expression and role of miR-17 in radioresistant cells of non-small cell lung cancer [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(5): 391-397.
[9]	CHEN Lin, WANG Enxiang, LIU Qunyou, ZHONG Ying, ZHOU Guoliang, ZHAO Xiyan. CDX2 gene inhibits of colon cancer cell proliferation by NF-κB signaling pathway [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(5): 398-403.
[10]	JIANG Liangliang, WANG Jing. Progress in diagnosis and treatment of distant metastasis of epithelial ovarian cancer [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(5): 480-482.
[11]	CHEN Zhaohong,HE Yingying,ZHANG Youcai,LU Dingyu. Effects of SOX7 overexpression on proliferation and other biological characteristics of gallbladder carcinoma GBC-SD cells and its mechanism [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(4): 293-297.
[12]	FAN Ningning, GENG Jingshu. The expression and role of LncRNA MEG3 in human glioma cells [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(3): 219-223.
[13]	DUAN Yanjun, XU Wei, CHEN Wenjie, WANG Fengjuan. Effects of Wnt pathway inhibitor ETC-159 on proliferation and migration of OSCC cells [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(2): 103-106.
[14]	BI Qiling, ZHOU Lihong, HAN Yimin. Research progress of HOX gene and gynecologic tumor [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(2): 140-143.
[15]	WANG Xiaoxiao, HUA Teng, WANG Hongbo. Research progress of estrogen receptor-related receptor γ in tumors [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(1): 87-91.

Effect of microRNA-150 on proliferation,apoptosis,invasion and metastasis of epithelial ovarian cancer cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments