PRACTICAL ONCOLOGY JOURNAL ›› 2019, Vol. 33 ›› Issue (3): 239-243.doi: 10.11904/j.issn.1002-3070.2019.03.009

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The expression of WEE1 in gastric cancer and its influence on prognosis of patients

MENG Hai1,DU Guanxiang1,ZHU Liling1,TANG Qiyun2   

  1. 1.Department of Gastroenterology,Binhai People′s Hospital,Yancheng 224500,China;
    2.Department of Geriatric Gastroenterology,Jiangsu People′s Hospital
  • Received:2018-12-11 Revised:2019-01-04 Online:2019-06-20 Published:2019-06-18

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Abstract: Objective The aim of this study was to investigate the expression of WEE1 in gastric cancer and its influence on prognosis of patients.Methods Seventy-eight patients with gastric cancer were enrolled.According to the presence or absence of lymph node(LN)metastasis,the patients were divided into LN(-)group and LN(+)group.The expression of WEE1 in gastric cancer tissues was detected by immunohistochemistry and RT-qRCR techniques.The influence of WEE1 expression on LN metastasis and prognosis of gastric cancer were analyzed statistically.Results (1)The positive rate of WEE1 in gastric cancer was 43.6%,of which 13 cases were a low expression and 21 cases with high expression.The positive rates of WEE1 in LN(+)group and LN(-)group were 53.8% and 23.1%,respectively.(2)The results of RT-qPCR showed that the mRNA expression of WEE1 in LN(-)and LN(+)groups was(1.32±0.21)and(3.64±0.41),respectively,and the difference was statistically significant(P＜0.01).(3)The results of ROC curve showed that the area under curve(AUC)of WEE1 mRNA expression level for LN metastasis of gastric cancer was 0.806,the sensitivity was 84.8%,the specificity was 79.6%,and the diagnostic efficacy was good.T stage and LN metastasis were independent risk factors for the expression of WEE1(P＜0.05).(4)Among 78 patients,7 patients were lost to follow-up,the rate of loss of follow-up was 8.9%,and 41 patients died within 5 years.Among them,13,10 and 18 died in the WEE1(-)group,low-expression of WEE1 group and high-expression of WEE1 group,respectively.The 5-year survival rates of the three groups were significantly different(χ2=25.67,P＜0.001).The survival rate in the low expression of WEE1 group was not significantly different from the high expression of WEE1 group(P＞0.05),but both of them were significantly low the WEE1(-)group(P＜0.05).Conclusion WEE1 has a high positive rate in gastric cancer,and is closely related to patient stage and LN metastasis.The positive expression of WEE1 is a strong signal for poor prognosis.

Key words: Gastric cancer, WEE1, Lymph node metastasis

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