Abstract:

Objective To identify the precise ultrastructural localization and difference of β-glucuronidase between the breast duct epithelial cells and the cells of invasive ductal carcinoma.Methods Low-temperature embedding,colloid gold labeling,and immunoelectron microscopy were performed,and the location of β-glucuronidase was observed under transmission electron microscope.Results The gold-particle-labeling β-glucuronidase was distributed in endoplasmic reticulum and lysosome.We found that the quantity of golden granules labeled β-glucuronidase in endoplasmic and lysosome of carcinoma cells was more than that in normal breast duct epithelial cells.Conclusion The morphological model of β-glucuronidase was established for further study in diseases,especially in neoplasms.

Key words: β-Glucuronidase, Colloid gold, Immunoelectron microscope, Breast duct epithelial cells, Invasive ductal carcinoma cells