PRACTICAL ONCOLOGY JOURNAL ›› 2012, Vol. 26 ›› Issue (1): 37-41.doi: 10.3969/j.issn.1002-3070.2012.01.007

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To Explore the Activity of Generation and the Cell Cycle of NCI-H460 by RNAi Inhibiting Bcl-2 Gene Expression and Combining Treatment with Radiation

XU Xiangying1,2,XU Dequan1,CHEN Mi1,ZHAO Fangzong1,HU Songliu1,XU Jianyu1.   

  1. 1.Department of radiotherapy,The Third Affiliated Hospital of Harbin Medical University,Harbin 150081;
    2.Harbin Medical University Institute of Cancer Prevention and Control,Harbin 150081
  • Received:2011-11-04 Online:2012-02-28 Published:2015-01-08

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Abstract: Objective To explore the influence of Bcl-2 gene expression by RNAi on cell cycle and activity of cell and the radiosensitivity of Non-Small cell lung cancer cell line NCI-H460.Methods Making use of pCYU6/GFP/Neo on vectors containing shRNA targeting Bcl-2 gene were transfected into NCI-H460.Western blot were used to compare the efficiency of gene silencing.The transfected cell were exposed to 6Gy X-rays generated by a linear accelerator.MTT and Flow cytometry were used to explore the activity of generation and the cell cycle of NCI-H460.Results Bcl-2/shRNA was successfully transfected NCI-H460 cell lines.Western blot analysis that expression of Bcl-2 was suppressed by Bcl-2/shRNA;The activity of generation of cells transfected with Bcl-2/shRNA was lower than others at 6Gy irradiation,and higher percentage of G2/M phase was observed on cells transfected with Bcl-2/shRNA than others by cytometry.Conclusion RNAi could inhibit Bcl-2 gene expression and enhance the activity of generation and the cell cycle of NCI-H460 cells.

Key words: Lung carcinoma, Bcl-2 gene, RNA interference, Cytometry

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