Abstract: Since 1970s,a Chinese study group at Harbin Medical University First Hospital began to use arsenic trioxide(As₂O₃) treatment of acute promyelocytic leukemia(APL) and achieved good results.Anticancer effect of As₂O₃ has become a research hotspot.In recent years,a growing number of studies demonstrate that glutathione(GSH) plays a central role in the delicate balance in determining sensitivity towards apoptosis or resistance of tumor cells.Cancer cells sensitive to As₂O₃ may contain less GSH than resistant cancer cells,and ascorbic acid(AA) can reduce the level of intracellular GSH.To improve anticancer effect of As₂O₃ and reduce its application dose,some scholars have proposed a new type of treatment program that As₂O₃ combination with AA for cancer treatment.In some basic experimental and clinical cancer research,As₂O₃ combination with AA has achieved some success.In light of this dosage regimen for the different tumors in the latest research progress,we conducted a review in this paper,which may be helpful for further exploring more reasonable chemotherapy regimens.