Abstract: Objective To investigate the expressions of transcription factor Sp1 and Survivin in colon cancer and their relationship between biological behavior and Survival rate.Methods Immunohistochemical techniques were used to detect the gene expressions of Sp1 and Survivin in 60 colon cancer specimens.Results The positive expression rates of Sp1 and Survivin in colon carcinoma were 45% and 53.3%,respectively.The expression of Suvivin had positive relation with the expression of Sp1.There was significant correlation among the expression of Sp1,tumor size,growth pattern,TNM stage and Dukes stage(P＜0.05).The expression levels of Survivin were correlated with cell differentiation,metastatic lymph nodes,TNM stage and Dukes stage(P＜0.05).With variable and multiple variable analysis,Sp1 and Survivin expressions and Dukes stage were independently prognostic factors of poor survival.Conclusions Sp1 and Survivin expressions may serve as valuable indicators of prognosis in colon carcinoma.