Abstract: Objective The aim of this study was to investigate the expression of COL5A2 in bladder cancer tissues,and its correlation with clinicopathological features and prognosis.Methods A total of 144 patients with bladder cancer were enrolled in this study.Real-time fluorescence reverse transcription and immunohistochemistry were used to detect the expression of COL5A2 at levels of mRNA and protein in bladder cancer tissues and normal bladder tissues.The relationship between COL5A2 expression and clinicopathological features and prognosis was analyzed.Results The expression of COL5A2 mRNA in the bladder cancer group was higher than that in the paracancerous group(P＜0.05).The positive rate of COL5A2 in the bladder cancer group was higher than that in the normal bladder tissues(P＜0.05).The expression of COL5A2 protein was not correlated with age(P＞0.05),positively associated with the TMN stage,pathological grade,tumor maximum diameter(≥5cm),depth of invasion,lymph node metastasis,lymphatic vascular infiltration,and recurrence;the difference was statistically significant(P＜0.05).The 3-year survival rate and survival time in the COL5A2 negative group were significantly higher than those in the COL5A2 positive group(P＜0.05).The higher TMN stage,the higher pathological stage,the maximum diameter of the tumor(≥5cm),the deeper infiltration depth,lymph node metastasis,lymphatic vascular infiltration,recurrence,the higher positive expression rate of COL5A2 protein.Conclusion COL5A2 is highly expressed in the bladder cancer tissues,which promotes the development of bladder cancer.Bladder cancer patients with low expression of COL5A2 can obtain a good prognosis.

Key words: COL5A2, Bladder cancer, Clinicopathological features, Prognosis