Abstract: Objective To observe the function of calcium sensing receptor(CaSR)in differentiated THP-1(human acute monocytic leukemia cell line)cells.Methods In the present study,we induced and differentiated the monocyte-like THP-1 cells into macrophage-like THP-1 cells with PMA.The secretion of cytokines was analyzed by ELISA.The expression of CaSR and NF-κB in the differentiated THP-1 cells was identified by Western blotting.Results (1)We found that GdCl₃ elevation stimulated both of IL-1β and TNFα release in differentiated THP-1 cells.(2)GdCl₃ could increase the expression of NF-κB-p65 and decrease the expression of NF-κB-IκBα.The effects of GdCl₃ were inhibited by NPS2390.Conclusion The activation of CaSR in macrophage-like THP-1 cells could commit to cytokine secretion via NF-κB signal transduction pathway.These results suggested that CaSR might be involved in a variety of pathological processes mediated by activated monocyte-macrophages.