Abstract: Objective To investigate the growth properties of human breast cancer cell line MCF7-pAcGFP labelled with green fluorescent protein.Methods Human breast cancer cell lines MCF-7 were transfected with pAcGFP-C1 by Fugene HD Transfection Reagent.The cells which express the pAcGFP gene were selected by geneticin(G418).The growth curve were detected by MTT,the cell cycle were detected by flow cytometry,and the expressions of 11 proteins in MCF7-pAcGFP cells were detected by western blot.Results The growth curve and cell cycle of MCF7-pAcGFP cells were similar to untransfected ones(P＞0.05).There were no significant diffrence in the expression of Cyclin D1,p27,Fas,FasL,Bcl-2,Bax,Akt,p-Akt(Ser473),p-Akt(Thr308),ERK and p-ERK between the MCF7-pAcGFP cells and MCF-7 cells.Conclusion Compared with parents cells,there was no significant change in growth properties of MCF7-pAcGFP cells.So MCF7-pAcGFP cells may provide a basis for construction a ideal animal model to research molecular mechanisms of growth in human breast cancer.