关键词: 重组人纤维连接蛋白, 细胞因子诱导的杀伤细胞, 增殖, 杀伤活性

Abstract:

Objective To investigate the recombinant human fibronectin(RetroNectin,RN)on proliferation,immunological characteristics and cytotoxicity of cytokine-induced killer cells(CIK).Methods Peripheral blood mononuclear cells(PBMCs)were cultured in vitro by precoating with RetroNectin(R-CIK group),CD3Ab(C-CIK group),RetroNectin combined with CD3Ab(R+C-CIK group)and traditional method(CIK group)were to generate CIK.The changes of growth rate,characterization,cytotoxicity and apoptosis of CIK were determined by cell counting,LDH assay and flow cytometry respectively.Results R+C-CIK cell showed a higher proliferation rate than other three groups.The difference was statistically significant(P＜0.05);The percentage of CD3/CD56 double positive cells in R+C-CIK and R-CIK group had a higher proportion than those in C-CIK and CIK group at day12 and day15(P＜0.05);Cytotoxicity of CIK towards K562 cells in R+C-CIK group and R-CIK group were significantly higher than that in C-CIK and CIK group,when effector-to-target ratio was 20∶1 and 40∶1(P＜0.05).We also found that the cytotoxicity of CIK towards LOVO cells in R+C-CIK group and R-CIK group were higher than that in C-CIK group and CIK group(P＜0.05).Conclusion RetroNectin and Anti-CD3-MAb synergistically promote antitumor efficiency of CIKs by increasing proliferation and cytotoxicity.

Key words: RetroNectin, CIK, Proliferation, Cytotoxicity