三阴性乳腺癌中miRNA-124表达与E-钙黏蛋白及雄激素受体的关系

doi:10.11904/j.issn.1002-3070.2017.02.001

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (2): 97-101.doi: 10.11904/j.issn.1002-3070.2017.02.001

• 基础研究 • 下一篇

三阴性乳腺癌中miRNA-124表达与E-钙黏蛋白及雄激素受体的关系

汤大北, 金艳, 龚振琴, 初众, 曹仲茹, 张清媛

哈尔滨医科大学附属肿瘤医院(哈尔滨 150081)

收稿日期:2016-10-27 出版日期:2017-04-20 发布日期:2017-05-04
通讯作者: 张清媛,E-mail:doctortang2008@126.com
作者简介:汤大北,女,(1983-),博士,副主任医师,从事乳腺癌诊疗的研究
基金资助:
黑龙江省教育厅科学技术研究项目(12541507)

Expression of miR-124 and its relationship with E-cadherin and androgen receptor in triple-negative breast cancer

TANG Dabei,JIN Yan,GONG Zhenqin,CHU Zhong,CAO Zhongru,ZHANG Qingyuan

Harbin Medical University Cancer Hospital,Harbin 150081,China

Received:2016-10-27 Online:2017-04-20 Published:2017-05-04

摘要/Abstract

摘要： 目的研究三阴性乳腺癌(Triple-negative breast cancer,TNBC)中miRNA-124(miR-124)的表达与E-钙黏蛋白(E-cadherin)及雄激素受体(Androgen receptor,AR)的关系。方法采用RT-PCR在TNBC组织和癌旁正常乳腺组织中检测miR-124的表达,免疫组化检测TNBC中E-cadherin及雄激素受体(AR)的表达。结果与癌旁正常乳腺组织相比,TNBC组织中miR-124表达明显降低(P＜0.05);TNBC组织中,miR-124表达与组织分级、E-cadherin表达有关(P＜0.05),与AR表达无关(P＞0.05)。结论在TNBC中,miR-124可能通过调节E-cadherin的表达发挥抑癌作用。

关键词: 三阴性乳腺癌, miR-124, E-钙黏蛋白, 雄激素受体

Abstract: Objective The objective of this study was to explore the expression of miRNA-124(miR-124)and its correlation with E-cadherin and androgen receptor(AR)in triple-negative breast cancer(TNBC).Methods The expression of miR-124 was detected by RT-PCR in TNBC tissues and adjacent normal breast tissues.The expression of E-cadherin and AR in TNBC was detected by immunohistochemistry.Results The expression of miR-124 in TNBC tissues was significantly correlated with histological grade and the expression of E-cadherin(P＜0.05),and the expression of miR-124 in TNBC tissues was significantly lower than that in adjacent normal breast tissues.No correlation was found between miR-124 and AR in TNBC tissues(P＞0.05).Conclusion In TNBC,miR-124 may play an anti-tumor effect by modulating the expression of E-cadherin.

Key words: Triple-negative breast cancer, miR-124, E-cadherin, Androgen receptor

中图分类号:

R37.9

汤大北, 金艳, 龚振琴, 初众, 曹仲茹, 张清媛. 三阴性乳腺癌中miRNA-124表达与E-钙黏蛋白及雄激素受体的关系[J]. 实用肿瘤学杂志, 2017, 31(2): 97-101.

TANG Dabei,JIN Yan,GONG Zhenqin,CHU Zhong,CAO Zhongru,ZHANG Qingyuan. Expression of miR-124 and its relationship with E-cadherin and androgen receptor in triple-negative breast cancer[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(2): 97-101.

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三阴性乳腺癌中miRNA-124表达与E-钙黏蛋白及雄激素受体的关系

Expression of miR-124 and its relationship with E-cadherin and androgen receptor in triple-negative breast cancer

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