过表达CLPTM1L降低95-D肺癌细胞对吉西他滨的敏感性

doi:10.11904/j.issn.1002-3070.2019.06.002

实用肿瘤学杂志 ›› 2019, Vol. 33 ›› Issue (6): 486-490.doi: 10.11904/j.issn.1002-3070.2019.06.002

过表达CLPTM1L降低95-D肺癌细胞对吉西他滨的敏感性

孙亦鹏, 倪振华, 吴颖颖, 陈清阁, 毕俊杰, 林玉华, 王雄彪

上海中医药大学附属普陀医院(上海 200333)

收稿日期:2019-08-21 修回日期:2019-09-03 发布日期:2019-12-28
通讯作者: 王雄彪,E-mail:xiongbiao6@hotmail.com
作者简介:孙亦鹏,男,(1990-),博士在读,住院医师,从事肺癌发病机制的研究。
基金资助:
上海市科学技术委员会科研计划项目(编号:18140904000;17401970900)

Overexpression of CLPTM1L inhibits the sensitivity of 95-D lung cancer cells to gemcitabine

SUN Yipeng, NI Zhenhua, WU Yingying, CHEN Qingge, BI Junjie, LIN Yuhua, WANG Xiongbiao

Department of Respiratory Diseases,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200333,China

Received:2019-08-21 Revised:2019-09-03 Published:2019-12-28

摘要/Abstract

摘要： 目的研究CLPTM1L基因与肺癌细胞对吉西他滨敏感性的关系,并探讨其潜在的作用机制。方法构建针对CLPTM1L基因的过表达慢病毒,并感染肺癌95-D细胞,将细胞分为CLPTM1L过表达组和对照组;采用CCK-8法检测吉西他滨处理后过表达组和对照组细胞增殖的变化;采用荧光定量PCR、Western blot和免疫化学发光法检测CLPTM1L基因和蛋白变化;采用生物荧光法检测Caspase-3/7和Caspase-9活性的变化;采用Western blot检测p-4E-BP1蛋白变化。结果 CLPTM1L过表达慢病毒感染95-D细胞后,CLPTM1L过表达组的CLPTM1L基因水平(P=0.036)和蛋白表达(P＜0.01)均高于对照组;与对照组相比,吉西他滨处理后CLPTM1L过表达组细胞增殖显著上升(P＜0.01);Caspase活性检测显示,CLPTM1L过表达组Caspase-3/7和Caspase-9活性较对照组明显下降(P＜0.01);Western blot检测显示,CLPTM1L过表达组4E-BP1的磷酸化水平与对照组比较显著增高(P＜0.01)。结论 CLPTM1L过表达可降低肺癌细胞对吉西他滨的敏感性,其机制可能是增加了4E-BP1的磷酸化水平。

关键词: 肺癌, CLPTM1L, 吉西他滨, p-4E-BP1

Abstract: Objective This study aimed to investigate the relationship between CLPTM1L gene and lung cancer 95-D cells sensitivity to gemcitabine,and to explore its potential mechanism of action.Methods Overexpression of lentivirus against CLPTM1L gene was constructed and infected with lung cancer 95-D cells;Cells were divided into the CLPTM1L overexpression group and control group;The proliferation of cells in the overexpressing and control groups after gemcitabine treatment was detected by CCK-8;The changes of CLPTM1L gene and protein were detected by real-time PCR,Western blot and immunochemiluminescence;The changes of caspase-3/7 and caspase-9 activities were detected by bioluminescence;Western blot was used to detect the changes of p-4E-BP1 protein.Results The expression of CLPTM1L gene(P=0.036)and its protein(P＜0.01)was significantly increased after CLPTM1L overexpressed lentivirus-infected 95-D cells;Compared with the control group,the proliferation of CLPTM1L overexpressing group after gemcitabine treatment was increased(P＜0.01);The activity of caspase activity showed that the activities of caspase-3/7 and caspase-9 in the CLPTM1L overexpression group were significantly lower than those in the control group(P＜0.01);The phosphorylated level of 4E-BP1 protein in the CLPTM1L overexpression group was significantly higher than that in the control group.Conclusion Overexpression of CLPTM1L can reduce the sensitivity of lung cancer cells to gemcitabine.Its mechanism may be to increase the phosphorylation level of 4E-BP1.

Key words: Lung cancer, CLPTM1L, Gemcitabine, p-4E-BP1

中图分类号:

R734.1

孙亦鹏, 倪振华, 吴颖颖, 陈清阁, 毕俊杰, 林玉华, 王雄彪. 过表达CLPTM1L降低95-D肺癌细胞对吉西他滨的敏感性[J]. 实用肿瘤学杂志, 2019, 33(6): 486-490.

SUN Yipeng, NI Zhenhua, WU Yingying, CHEN Qingge, BI Junjie, LIN Yuhua, WANG Xiongbiao. Overexpression of CLPTM1L inhibits the sensitivity of 95-D lung cancer cells to gemcitabine[J]. Journal of Practical Oncology, 2019, 33(6): 486-490.

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链接本文: http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/10.11904/j.issn.1002-3070.2019.06.002

http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2019/V33/I6/486

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过表达CLPTM1L降低95-D肺癌细胞对吉西他滨的敏感性

Overexpression of CLPTM1L inhibits the sensitivity of 95-D lung cancer cells to gemcitabine

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