circRNA MYO1C对肺癌细胞自噬和肿瘤干细胞分化的影响

doi:10.11904/j.issn.1002-3070.2021.06.003

实用肿瘤学杂志 ›› 2021, Vol. 35 ›› Issue (6): 500-505.doi: 10.11904/j.issn.1002-3070.2021.06.003

circRNA MYO1C对肺癌细胞自噬和肿瘤干细胞分化的影响

罗雯¹, 贾莉², 张佳文², 王冬杰², 孔英君¹

1.深圳市第三人民医院国家感染性疾病临床医学研究中心,南方科技大学第二附属医院呼吸内科(深圳 518055);
2.哈尔滨医科大学附属第一医院呼吸与危重症医学科

收稿日期:2021-03-30 修回日期:2021-10-27 出版日期:2021-12-28 发布日期:2021-12-17
通讯作者: 孔英君,E-mail:kyjtoday@163.com
作者简介:罗雯,女,(1983-),博士,副主任医师,从事肺癌早期诊断与治疗的研究。

The effects of circRNA MYO1C on lung cancer cell autophagy and cancer stem cell differentiation

LUO Wen¹, JIA Li², ZHANG Jiawen², WANG Dongjie², KONG Yingjun¹

1. Respiratory Medicine Department,National Clinical Research Center for Infectious Disease,Shenzhen Third People′s Hospital,The Second Affiliated Hospital,School of Medicine,Southern University of Science and Technology,Shenzhen 518000,China;
2. Department of Respiratory and Critical Care Medicine,The First Affiliated Hospital of Harbin Medical University

Received:2021-03-30 Revised:2021-10-27 Online:2021-12-28 Published:2021-12-17

摘要/Abstract

摘要： 目的探讨环状RNA(circRNA)MYO1C对非小细胞肺癌(NSCLC)细胞自噬和肿瘤干细胞(Cancer stem cells,CSCs)分化的影响。方法通过RT-qPCR检测正常肺上皮细胞和NSCLC A549细胞中circRNA MYO1C水平。将A549分为对照组、NC组和siMYO1C组,通过转染si-circRNA MYO1C质粒干扰circRNA MYO1C。用CCK-8法和Transwell实验检测细胞生长活力和迁移能力。通过Western blot检测CSCs分化标志蛋白(OCT4、SOX2和NANOG)以及自噬标志蛋白(P62和IC3II/LC3I)的表达水平。结果相对正常肺上皮细胞,circRNA MYO1C在A549细胞中表达明显上调(0.99±0.06 vs. 4.87±0.88,P=0.002)。与NC组比较,siMYO1C组细胞中circRNA MYO1C水平、细胞活力、迁移、OCT4、SOX2、NANOG和IC3II/LC3I蛋白水平显著降低,而P62蛋白水平显著升高(P＜0.001)。结论下调circRNA MYO1C能够抑制NSLCLC细胞的CSCs分化和自噬相关蛋白表达,并抑制肺癌细胞迁移。

关键词: 非小细胞肺癌, 环状RNA MYO1C, 肿瘤干细胞分化, 自噬

Abstract: Objective The aim of this study was to investigate the effects of circRNA MYO1C on autophagy and cancer stem cells(CSCs) differentiation of non-small cell lung cancer(NSCLC). Methods The levels of circRNA MYO1C in normal lung epithelial cells and NSCLC A549 cells were detected by RT-qPCR.A549 cells were divided into the control,NC and siMYO1C groups,and circRNA MYO1C was interfered by transfection with si-circRNA MYO1C plasmids.The cell growth viability and migration ability were detected by CCK-8 and Transwell assays.The expression of CSCs differentiation marker proteins(OCT4,SOX2 and NANOG)and autophagy marker proteins(P62 and IC3II/LC3I)was detected by Western blot. Results Compared with normal lung epithelial cells,the expression of circRNA MYO1C was significantly up-regulated in A549 cells(0.99±0.06 vs. 4.87±0.88,P=0.002).Compared with the NC group,the level of circRNA MYO1C,cell viability,cell migration,the levels of OCT4,SOX2,NANOG and IC3II/LC3I proteins in the siMYO1C group were significantly reduced,the level of P62 protein was significantly increased(P＜0.001). Conclusion Down-regulating circRNA MYO1C can inhibit CSCs differentiation and autophagy-related protein expression of NSLCLC cells,and inhibit lung cancer cell migration.

Key words: Non-small cell lung cancer, circRNA MYO1C, Cancer stem cells differentiation, Autophagy

中图分类号:

R734.2

罗雯, 贾莉, 张佳文, 王冬杰, 孔英君. circRNA MYO1C对肺癌细胞自噬和肿瘤干细胞分化的影响[J]. 实用肿瘤学杂志, 2021, 35(6): 500-505.

LUO Wen, JIA Li, ZHANG Jiawen, WANG Dongjie, KONG Yingjun. The effects of circRNA MYO1C on lung cancer cell autophagy and cancer stem cell differentiation[J]. Journal of Practical Oncology, 2021, 35(6): 500-505.

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http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2021/V35/I6/500

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circRNA MYO1C对肺癌细胞自噬和肿瘤干细胞分化的影响

The effects of circRNA MYO1C on lung cancer cell autophagy and cancer stem cell differentiation

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