关键词: LINC00511, miR-150-5p, 聚梳组蛋白家族指蛋白1, 结直肠癌, 上皮-间充质转化, 增殖, 凋亡

Abstract: Objective The aim of this study was to investigate the effects of LINC00511 on proliferation,apoptosis,and epithelial mesenchymal transition(EMT)of colorectal cancer cells regulating by the miR-150-5p/PHD family finger protein 1(PHF1)axis. Methods The tumor tissues and adjacent tissues were collected from 24 colorectal cancer patients who underwent surgical treatment in our hospital from March 2021 to March 2023.Colorectal cancer SW480 cells were cultured,and randomly separated into the normal control group(Ctrl group),si-NC group,si-LINC00511 group,si-LINC00511+inhibitor NC group,and si-LINC00511+miR-150-5p inhibitor group.RT-qPCR was used to detect the expression of LINC00511,miR-150-5p,and PHF1 mRNA in cancer tissues and adjacent tissues of colorectal cancer patients and SW480 cells.CCK-8 method was used to detect the proliferative ability in SW480 cells.Transwell experiment was used to detect cell invasion and migration abilities.Flow cytometry was applied to detect cell apoptosis.Immunoblotting was used to detect the expression of PHF1,apoptosis,and proteins related to EMT.The dual luciferase reporter gene experiment verified the relationship between LINC00511 and miR-150-5p,or between miR-150-5p and PHF1,respectively. Results Compared with adjacent tissues,the expression of LINC00511 and PHF1 mRNA increased in the tumor tissues of colorectal cancer patients,while the expression of miR-150-5p decreased(P＜0.001).Compared with the si-NC group,the expression of LINC00511,cell proliferation(OD₄₅₀ value),numbers of cell migration and invasion,and the expression of Bcl-2,PHF1,N-cadherin,and vimentin proteins in SW480 cells were significantly reduced in the si-LINC00511 group(P＜0.01),while miR-150-5p,cell apoptosis rate,the expression of Bax and E-cadherin proteins were significantly increased(P＜0.001).Compared with the si-LINC00511+inhibitor NC group,the changes in corresponding indicators of SW480 cells in the si-LINC00511+miR-150-5p inhibitor group were opposite to those mentioned above(P＜0.001).LINC00511 had targeted negative regulation of miR-150-5p expression,while miR-150-5p targeted negative regulation of PHF1 expression. Conclusion Knockdown of LINC00511 may promote the expression of miR-150-5p,thereby inhibiting the expression of PHF1,suppressing colorectal cancer cell proliferation and EMT,and inducing apoptosis of colorectal cancer cells.

Key words: LINC00511, MiR-150-5p, PHD family finger protein 1, Colorectal cancer, Epithelial-mesenchymal transition, Proliferation, Apoptosis