血清长链非编码RNA MALAT1和AFAP1-AS1与鼻咽癌临床病理特征和预后的关系

doi:10.11904/j.issn.1002-3070.2019.06.007

Abstract

Abstract: Objective The objective of this study was to investigate the relationship between serum long-chain non-coding RNA MALAT1 and AFAP1-AS1 and clinicopathological features,and prognosis of nasopharyngeal carcinoma(NPC).Methods A total of 136 patients with nasopharyngeal carcinoma confirmed by pathology in our hospital were selected from April 2013 to June 2015.During the same time,54 outpatients for health examination in our hospital were selected as the control group and nasopharyngeal carcinoma group.Real-time fluorescence reverse transcription analysis was used to analyze the expression of lncRNA MALAT1 and AFP1-AS1.The relationship between the expression of lncRNA MALAT1 and AFP1-AS1,and clinicopathological characteristics was analyzed by χ² test.Log-rank assay was used to analyze serum long-chain non-coding RNA MALAT1 and prognostic differences in patients with different expression levels of AFAP1-AS1.Results Compared with the control group,the serum levels of RNA MALAT1 and RNA AFAP1-AS1 in the nasopharyngeal carcinoma group were significantly increased(P＜0.001);The expressions of lncRNA MALAT1 and AFAP1-AS1 was not related to age(P＞0.05);The maximum diameter of the tumor was ≥5 cm,the pathological stage was higher,the TNM stage was higher,the deeper in the infiltration depth,the infiltration of lymphatic vessels,the lymph node metastasis,and the recurrence and the higher in high expressive rates of lncRNA MALAT1 and AFAP1-AS1(P＜0.05).The 2-year survival rate and survival time of lncRNA MALAT1 and AFAP1-AS1 in the low expression group were significantly higher than those of the high expression group(P＜0.001);Multivariate Cox stepwise regression analysis showed that low expression of lncRNA MALAT1(HR=0.52,95% CI:0.37~0.81)and low expression of lncRNA AFAP1-AS1(HR=0.56,95% CI:0.51~0.83)were independent prognostic protective factors for NPC patients(P＜0.001).Conclusion The serum long-chain non-coding RNA MALAT1 and AFAP1-AS1 are elevated in patients with NPC,and are positively correlated with malignant progression of NPC.NPC patients with low expression of lncRNA MALAT1 and AFAP1-AS1 serum have a good prognosis;MALAT1 and AFAP1-AS1 can be used as new markers for the diagnosis of nasopharyngeal carcinoma.

Key words: Serum long non-coding RNA MALAT1, Serum long non-coding RNA AFAP1-AS1, Nasopharyngeal carcinoma, Clinicopathological features, Prognosis

CLC Number:

R739.62

LIU Gengchun, SHEN Liangfang. Relationship between serum long-chain non-coding RNA MALAT1 and AFAP1-AS1,and clinicopathological features and prognosis of nasopharyngeal carcinoma[J]. Journal of Practical Oncology, 2019, 33(6): 513-518.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2019.06.007

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2019/V33/I6/513

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Relationship between serum long-chain non-coding RNA MALAT1 and AFAP1-AS1,and clinicopathological features and prognosis of nasopharyngeal carcinoma

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