lncRNA DUXAP8在子宫内膜癌细胞中的作用机制研究

doi:10.11904/j.issn.1002-3070.2023.02.008

Abstract

Abstract: Objective The aim of this study was to explore the role of lncRNA DUXAP8 in the occurrence and development of endometrial cancer(EC). Methods The Cancer Genome Atlas(TCGA)database was used to analyze the expression level of lncRNA DUXAP8 in EC tissues and the association between DUXAP8 and prognosis of patients.qRT-PCR was used to detect the expression of DUXAP8 in EC cells lines.HEC-1A cell lines with DUXAP8 overexpressed or knockdown were constructed by transfection.CCK-8,colony forming,transwell,scratch healing assay,and flow cytometry were used to detect the abilities of proliferation,invasion,migration,cell cycle,and cell apoptosis in endometrial cancer cells after different treatments,and Western blot was used to detect the levels of epithelial-mesenchymal transition(EMT)-related protein. Results DUXAP8 was significantly upregulated in endometrial cancer,and endometrial cancer patients with high DUXAP8 expression had a relatively poor prognosis.Inhibition of DUXAP8 hinder cell proliferation,invasion,migration and EMT process,cell cycle arrested at the G0/G1 phase,and induced apoptosis.While overexpression DUXAP8 enhanced the proliferation,invasion,migration and EMT process of tumor cells,cell cycle arrested at the S phase,and apoptosis level significantly reduced. Conclusion These results suggested that DUXAP8 functions regard as a pro-oncogene in endometrial cancer and may be a new target for EC therapy.

Key words: Endometrial cancer, Double homeobox A pseudogene 8, Proliferation, Apoptosis

CLC Number:

R737.33

XIA Jianhong, ZHOU Liqing, YAN Yan, MA Tingting, SU Xinyu. The function and mechanism of lncRNA DUXAP8 in endometrial cancer cells[J]. Journal of Practical Oncology, 2023, 37(2): 137-143.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2023.02.008

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2023/V37/I2/137

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The function and mechanism of lncRNA DUXAP8 in endometrial cancer cells

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