PRACTICAL ONCOLOGY JOURNAL ›› 2010, Vol. 24 ›› Issue (1): 8-10.doi: 10.3969/j.issn.1002-3070.2010.01.002

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Study of expression efficiency and genetic stability of adenovirus recombined with hIL-2 and hGM-CSF gene in AGZY Cells

SHEN Baozhong1, LIU Ming1, TIAN Changfu2, LI Renfei1   

  1. 1.The Fourth Hospital of Harbin Medical University,Harbin 150001;
    2.The Cancer Institute of Harbin Medical University
  • Received:2010-01-15 Online:2010-02-28 Published:2015-01-23

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Abstract: Objective To study the expressing of recombinant adenovirus with exogenesis genes in AGZY and the genetic stability.Method Transfected AGZY with two kinds of rAd.Then,detect the mRNA expression of Gene hIL-2 and Gene hGM-CSF with half-quantity RT-PCR.Result The expression level of hIL-2 and hGM-CSF in AGZY increased with time upto 72h.Conclusion The AGZY passage has no affect on the expression efficiency and the genetic stability of rAd-IL、rAd-GM-CS.

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