Abstract: Objective To investigate the effect of D-limonene on the expressions of p-ERK and p-AKTs in human colon cancer cell line LS174T.Methods The effect of D-limonene on the cell proliferation was measured by methyl thiazolyl tetrazolium(MTT),and the expression of ERK1/2,Akt and their phosphorylated proteins(p-ERK and p-AKTs)were detected by Western blot.Results D-limonene inhibited the proliferation of LS174T cell in a dose dependent manner,because the LS174T cell were exposed to D-limonene for 48 hours.D-limonene did not influence the expressions of ERK1/2 and Akt,but it significantly decreased p-ERK1/2 and p-Akts expressions.Conclution It is suggested that D-limonene could inhibit proliferation of LS174T cell via inhibiting the p-ERK1/2 and p-Akts pathway.