Objective To study a retrospective analysis of the clinical effect of the neoadjuvant therapy of treating Her-2 positive breast cancer by applying Trastuzumab together with athracyclines and taxane-based chemotherapy.Methods 30 patients with Her-2 positive operable breast cancer were accepted 4 cycles ATH which were followed by 2~4 cycles TH regimen neoadjuvant chemotherapy before modified radical　mastectomy or conserving surgery.Then a statistical analysis was made.Results Among the 30 concerned patients,25(80%)reached completly remissio and Partial remission,and 15(50%)obtained pathological complete remission.Conclusion A complete pathological remission as well as a considerably high clinical benefit ratio can be achieved by treating Her-2 positive breast cancer with Trastuzumab together with athracyclines and taxane-based chemotherapy.Adverse reaction is tolerable.

Objective To investigate the effect of D-limonene on the expressions of p-ERK and p-AKTs in human colon cancer cell line LS174T.Methods The effect of D-limonene on the cell proliferation was measured by methyl thiazolyl tetrazolium(MTT),and the expression of ERK1/2,Akt and their phosphorylated proteins(p-ERK and p-AKTs)were detected by Western blot.Results D-limonene inhibited the proliferation of LS174T cell in a dose dependent manner,because the LS174T cell were exposed to D-limonene for 48 hours.D-limonene did not influence the expressions of ERK1/2 and Akt,but it significantly decreased p-ERK1/2 and p-Akts expressions.Conclution It is suggested that D-limonene could inhibit proliferation of LS174T cell via inhibiting the p-ERK1/2 and p-Akts pathway.

Objective To study the effect of Angiotensin Ⅱ on cell proliferation and the activity of matrix metalloproteinase in breast cancer cells.Methods MCF-7 cells were cultured in vitro and stimulated with Angiotensin Ⅱ and/or Losartan.The viability of cells was tested by MTT assay.We used RT-PCR to detect the mRNA level of MMP-2,MMP-9,TIMP-1 and TIMP-2.The activity of MMP-2 and MMP-9 were measured by gelatin zymography.Results Angiotensin Ⅱ stimulated the growth of breast cancer cells in a dose-and time-dependent manner.Moreover,the expressions of MMP-2 and MMP-9 mRNA were significantly up-regulated by Angiotensin Ⅱ.The expressions of TIMP-1 and TIMP-2 mRNA were significantly down-regulated by Angiotensin Ⅱ.In addition,Angiotensin Ⅱ significantly enhanced the activity of MMP-2 and MMP-9.Angiotensin Ⅱ had a maximal response with 100nM(P＜0.05).Losartan significantly decreased the effect of Angiotensin Ⅱ on matrix metalloproteinase.Conclusion In summary,our study can support the concept that Angiotensin Ⅱ/ Angiotensin Ⅱ type 1 receptor(AT1R)signaling can regulate the expression of matrix metalloproteinase.Losartan can significantly decrease the effect of Angiotensin Ⅱ on matrix metalloproteinase.Therefore,targeting Angiotensin Ⅱ/ Angiotensin Ⅱ type 1 receptor(AT1R)signaling could be a novel therapeutic for breast cancer.

Objective To discuss on the BALB/C of lung tissue in mice after irradiation,TNF-alpha and IL-1alpha release characteristics of cytokines in the lung and liver.Methods Irradiation of male BALB/C mouse 30 was randomly assigned to a group(R)and the control group(C).R group:24 / group,C group:6 / group,6MV-X -Ray 25Gy chest irradiation;two groups of mice after exposure to 1,and 2,and 3 months were executed using immunohistochemical Methods for detection of irradiation in mouse lung and liver tissue of TNF-α and IL-1α expression levels.Results R group of TNF-α and IL-1α expressions in lung and liver levels of exposure to 1,and 2,and 3 months of significantly higher C(P＜0.01)and 2 months after the expression of the highest.Conclusion Lung tissues after exposure TNF-α and IL-1α expressions in lung and liver levels are significantly increased.Exposure 2 months in mice TNF-α and IL-1α expressions are particularly significant.

Objective To determine the effect of phenolic alkaloids of menispermum dauricum on the gastric cancer cell SGC-7901 proliferation.Methods We studied the effect of phenolic alkaloids of menispermum at different concentrations and different times on the gastric cancer cell SGC-7901 proliferation by MTT and trypan blue methods.Results PAMD can obviously inhibit SGC-7901 cell proliferation,and is dose-and time-dependent relationship with extended periods of concentration increase.Its role and inhibition rate was on the rise.Conclusion PAMD may have some therapeutic effect on gastric cancer.

Objective To summarize the etiology,treatment and preventive experience of the chylous fistula after radical resection of colon carcinoma plus D3 lymph node dissection.Methods 628 cases of the chylous fistula after radical resection of colon carcinoma plus D3 lymph node dissection were analyzed retrospectively.The incidence of the chylous fistula in different surgical methods(the Laparotomy Group and the laparoscopy group)and different surgical sites(left colon cancer and right colon cancer)was compared.Results Chylorrhea occurred in 22 patients(3.5%),including 2.8%(8/286)in the Laparotomy Group and 4.0%(14/342)in the Laparotomy Group,and the differences were no statistical significance(P＞0.05).Incidence of the chylous fistula in radical resection of right-colon carcinoma plus D3 lymph node dissection was 7.8%(16/207),while incidence of the chylous fistula in radical resection of right-colon carcinoma plus D3 lymph node dissection was 1.4%(6/421).There was statistical significance(P＜0.05),and there was no significant statistical significance between different TNM stages and incidence of the chylous fistula(P＞0.05).Twenty-one patients received conservative treatment(abrosia,total parenteral nutrition,somatostatin infusion,anti-infection,delaying removing drainage tubes)were successfully cured,and one patient required re-operation.No patients had recurrence and died.Conclusion There is no statistical significance between the Laparotomy Group and the laparoscopy group.Radical resection of right-colon carcinoma plus D3 lymph node dissection is associated with higher risk for chyle leak.Conservative treatment is effective in early stage of chyle leak after radical resection of colon carcinoma plus D3 lymph node dissection.

Objective To investigate the esophagus cancer mortality trend from 1985 to 2009 and project future ten years(2010—2019)in Henan province.Methods The data of esophagus cancer deaths and population data from 1985 to 2009 was drawn from Henan Provincial Center for Tumor and Vital Statistics Database.The mortality rates of periods and the age-standardized mortality rates by Chinese population(1982)and by world Segi′s population were calculated.Joinpoint regression model was used to estimate the time trends of the mortality.Using the Leslie matrix to predict the population of Henan province during 2010—2019.The cancer mortality during 2010—2019 in Henan province was projected by APC regression model.Results A total of 72294 cases which died from esophagus cancer were reported by vital statistics and registry center in Henan province from 1985 to 2009.Among male,the age-standardized mortality rates by Chinese population was 49.49 per 100,000 during period of 1985—1989 year and decreased to 17.72 per 100,000 during period of 2005—2009 year.For female,the age-standardized rates decreased from 26.48 per 100,000 to 9.06 per 100,000.The estimated trend parameters by Joinpoint model showed that the standardized-mortality rate of esophagus cancer by Chinese standard population showed the decreasing trend in both male and female.There are two joinpoint in male and two joinpoint in female.Predicted model demonstrated that the average rate of population increased in Henan province will be 4.38 ‰ during 2010—2019.The total population of the 15 cities and counties in Henan province in 2019 will reach 14.87 million.In the future,the age-standardized mortality rates by Chinese standard population in male and female will be 17.04 per 100,000 and 8.73 per 100,000 during 2010—2014,and those in male and female are 16.67 per 100,000 and 7.98 per 100,000 respectively during 2015—2019.Conclusion The mortality rates of esophagus cancer in Henan province showed the decreasing trend during the period of 2010—2019.But the mortality rate is still high.Prevention and control measures should be continued.

Objective To evaluate the risk factors related to the incidence of cervical cancer through a case-control study on the the comparison of cervical cancer patients in Shenyang with local residents in Shenyang.Methods In reference to the patients with cervical cancer hospitalized in Liaoning Tumor Hospital from Shenyang,1000 residents living in Ertaizi Street,Dadong District,Shenyang were randomly selected for the screening of cervical cancer and its precancerous lesions,the epidemiological investigations and the collections of HPV samples and cervical smear samples.The obtained data put into SPSS 13.0 statistical software to make the analysis of single-factor χ² analysis,logistic regression analysis and multi-factor logistic regression analysis.Results HPV positive(OR=221.62,95%CI:28.53~1721.51),the first sex life younger than 21 years old(OR=6.65,95%CI:1.88~23.51)is the risk factors of Cervical cancer;condom(OR=0.05,95%CI:0.01~0.18)is the protect factor of Cervical cancer.Conclusion It is of great significance to remove HPV infection,advocate the use of condoms,establish a scientific concept of sexual life and avoid early sexual experience in reducing the incidence of cervical cancer in Shenyang.

Objective To investigate endoscope image of malignant melanoma′s duodenum metastasis after operation.Methods We analysed the clinical date of a malignant melanoma patient after operation to clear diagnosis with endoscope and pathological examination.Results Malignant melanoma has strong metastasis and the endoscope image is similar to the mole of cutaeous.Conclusion Examination of endoscope combined with pathology plays an important role in diagnosis of upper digestive tract′s malignant melanoma.