EGFR,HER2和HER3在胆管癌中的表达及预后价值

doi:10.11904/j.issn.1002-3070.2019.06.003

Abstract

Abstract: Objective The aim of this study was to observe the expression of human epidermal growth factor receptor(EGFR),epidermal growth factor receptor 2(HER2)and epidermal growth factor receptor 3(HER3)in cholangiocarcinoma(CCA),and to explore the relationship between the expression of EGFR,HER2,HER3 and the clinicopathological features,overall survival time and proliferation of CCA patients.Methods Fifty patients with CCA who underwent surgery in our hospital from January 2009 to October 2013 were enrolled,and their adjacent tissues were also collected as controls.Immunohistochemistry and qRT-PCR were used to detect the expression of EGFR,HER2 and HER3 in CCA and adjacent tissues.The clinicopathological parameters of patients were collected and the relationship between EGFR,HER2,and HER3 expression and clinicopathological parameters of CCA was analyzed.Kaplan-Meier survival curves were plotted,and the relationship between the expression of EGFR,HER2,and HER3 and total postoperative survival of 50 patients with CCA was analyzed using the Log-rank test and Cox proportional hazard model.The expression of EGFR,HER2 and HER3 in CCA QBC939 cell line was knocked down by RNA interference assay.The knockdown effect of EGFR,HER2 and HER3 was detected by Western blot.The effect of EGFR,HER2 and HER3 on proliferation of QBC939 cells was determined by MTT assay.Results The positive expression of EGFR,HER2 and HER3 was determined in CCA tissues.The relationship analysis of clinicopathological characteristics showed that the HER2 expression was associated with CCA lymph node metastasis(P＜0.05).EGFR and HER3 was associated with CCA lymph node metastasis and correlated with cancer differentiation(P＜0.05).The overall survival of patients with EGFR,HER2 and HER3 positive was significantly lower than that of negative patients(P＜0.05).After knocking EGFR,HER2 and HER3 expression,the proliferation was significantly decreased in QBC939 cells(P＜0.05).Conclusion The expression of EGFR,HER2 and HER3 in CCA tissues is closely related to the overall survival of patients,and the mechanism may be related to the promotion of proliferation of CCA cells.

Key words: Epidermal growth factor receptor, Human epidermal growth factor receptor 2, Human epidermal growth factor receptor 3, Cholangiocarcinoma, Proliferation

CLC Number:

R735.8

ZHAN Qi, ZHOU Sheng, LOU Liping. The expression and prognostic value of EGFR,HER2 and HER3 in cholangiocarcinoma[J]. Journal of Practical Oncology, 2019, 33(6): 491-496.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2019.06.003

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2019/V33/I6/491

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The expression and prognostic value of EGFR,HER2 and HER3 in cholangiocarcinoma

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