NOX4在结肠癌组织中的表达及与预后的关系

doi:10.11904/j.issn.1002-3070.2020.05.002

Abstract

Abstract: Objective The Objective of this study was to investigate the expression of NADPH oxidase-4(NOX4)in colon cancer and to explore its relationship with the prognosis of colon cancer.Methods Eighty paraffin specimens of colon cancer patients treated in our hospital from January to December 2015 were selected for immunohistochemical staining.The COAD data set of colon cancer was obtained from the Cancer and Tumor Gene Atlas(TCGA)database to observe the expression of NOX4 in colon cancer and its prognostic value.Bioinformatics methods were used to analyze the genes and signal pathways related to NOX4.A nomogram was constructed to predict the prognosis of colon cancer patients,and the C index was used to evaluate the prediction effect of nomogram.According to the prognosis risk score,patients were divided into the high-risk group and the low-risk group,and the Kaplan-Meier method was used to compare the prognosis of the two groups.Results The expression of NOX4 in colon cancer tissues was significantly higher than that in normal intestinal mucosa tissues,and its expression level increased with the increase of cancer stage.The prognosis of patients with high expression of NOX4 was worse than those with low expression(P＜0.05).The results of gene enrichment analysis showed that NOX4 might be involved in the regulation of tumor-related signal pathways such as JAK-STAT,MAPK,TGF-β and WNT,and the production and secretion of interleukin-1 and interleukin-6.The results of Cox multivariate regression analysis showed that age,N stage,M stage,postoperative chemotherapy and the expression of NOX4 were independent risk factors affecting OS in colon cancer patients.These factors were used to establish a nomogram for predicting OS in patients with colon cancer,the C index was 0.735(internal validation)and 0.727(external validation).The calibration curve showed that the nomogram had better predictive performance.Conclusion The expression of NOX4 in colon cancer tissues is significantly increased,and it is an independent risk factor affecting the prognosis of colon cancer patients.The constructed prognosis model based on NOX4 has a good predictive power.

Key words: Colon neoplasm, NOX4, TCGA, Nomogram, Prognosis

CLC Number:

R735.3+5

LIU Hengchang, WEI Ran, LI Chunxiang, LIU Zheng, CHEN Haipeng, GUAN Xu, ZHAO Zhixun, ZOU Shuangmei, WANG Xishan, JIANG Zheng. Expression of NOX4 in colon cancer and its relationship with prognosis[J]. Journal of Practical Oncology, 2020, 34(5): 391-397.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2020.05.002

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2020/V34/I5/391

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Expression of NOX4 in colon cancer and its relationship with prognosis

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