牙髓干细胞外泌体对口腔鳞状细胞癌CAL-27增殖、迁移的影响及其机制的研究

doi:10.11904/j.issn.1002-3070.2022.05.002

Abstract

Abstract: Objective The objective of this study was to investigate the effects of human dental pulp stem cell derived exosomes (hDPSCs-exo) on the proliferation and migration of oral squamous carcinoma CAL-27 cells. Methods hDPSCs-exo was extracted by ultracentrifugation.CAL-27 cells were treated with different concentrations of hDPSCs-exo.The proliferation and migration of CAL-27 cells were detected by MTT assay,Transwell and scratch assays;Western Blot was used to detect the levels of PI3K/AKT signaling pathway related proteins. Results The results of MTT assay showed that only 80 μg/mL of hDPSCs-exo had an inhibitory effect on the proliferation of CAL-27 cells on the 5th day (P＜0.05),and the other concentration groups had no significant effect.The results of Transwell and scratch assays showed that 20,60,and 80 μg/mL of hDPSCs-exo could significantly promote migration of CAL-27 cells (P＜0.05).The results of Western blot showed that the levels of PI3K/AKT signaling pathway related proteins PI3K and P-AKT were significantly up-regulated in CAL-27 cells after hDPSCs-exo treatment(P＜0.05). Conclusion High concentration of hDPSCs-exo can inhibit the proliferation of CAL-27 cells,and the appropriate concentration can significantly promote the migration of CAL-27 cells.Its mechanism of action may be related to the activation of the PI3K/AKT signaling pathway.

Key words: Dental pulp stem cells, Exosomes, Oral squamous cell carcinoma, PI3K/Akt, Proliferation, Migration

CLC Number:

R739.8

YUE Jie, YANG Qilin, SUN Xiangyu, SUN Yuming, MA Xiang, CHEN Dong, JING Guangping. Effects of exosomes derived from dental pulp stem cells on the proliferation and migration of oral squamous cell carcinoma CAL-27 and its mechanism[J]. Journal of Practical Oncology, 2022, 36(5): 398-403.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2022.05.002

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2022/V36/I5/398

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Effects of exosomes derived from dental pulp stem cells on the proliferation and migration of oral squamous cell carcinoma CAL-27 and its mechanism

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