人参多糖注射液对人乳腺癌MCF-7细胞侵袭迁移能力的影响及机制研究

doi:10.11904/j.issn.1002-3070.2015.04.009

实用肿瘤学杂志 ›› 2015, Vol. 29 ›› Issue (4): 333-336.doi: 10.11904/j.issn.1002-3070.2015.04.009

人参多糖注射液对人乳腺癌MCF-7细胞侵袭迁移能力的影响及机制研究

罗莹¹ ,黄晶²

1.湖北省松滋市人民医院(松滋 434200);
2.武汉协和医院肿瘤中心

收稿日期:2015-01-30 出版日期:2015-08-27 发布日期:2015-08-27
通讯作者: 黄晶 E-mail:huangjing10h@163.com
作者简介:罗莹,女,(1981-),本科,主治医师,从事肿瘤内科、肿瘤放化疗方向的研究

The effect and mechanism of ginseng polysaccharides injective solution on invasive ability of human breast cancer MCF-7 cells

LUO Ying¹,HUANG Jing²

1.Department of Oncology,The First people′s Hospital of Songzi,Songzi 434200,China;
2.Cancer Center of Wuhan Union Hospital

Received:2015-01-30 Online:2015-08-27 Published:2015-08-27

摘要/Abstract

摘要： 目的探讨人参多糖注射液对人乳腺癌细胞株MCF-7侵袭迁移能力的影响。方法以不同作用浓度(12.5μL/mL、25μL/mL、50μL/mL、100μL/mL)人参多糖注射液处理MCF-7细胞株,以PBS作为空白对照,分别采用Transwell小室侵袭实验、划痕实验及Western blot检测细胞侵袭能力、迁移能力及基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)表达。结果人参多糖注射液对MCF-7细胞体外侵袭和迁移能力具有显著的抑制作用,并呈现剂量及时间依赖性。人参多糖注射液处理后,MCF-7细胞MMP-2蛋白表达及MMP-9蛋白表达比值显著降低,并呈现剂量及时间依赖性。结论人参多糖注射液可显著抑制人乳腺癌细胞MCF-7侵袭能力和迁移能力,下调MMP-2及MMP-9蛋白表达是其可能的作用机制。

关键词: 人参多糖注射液, 乳腺癌, 基质金属蛋白酶-2, 基质金属蛋白酶-9

Abstract: Objective To evaluate the effect and mechanism of ginseng polysaccharides injective solution on invasive ability of human breast cancer MCF-7 cells.Methods Human breast cancer MCF-7 cells were treated with different concentrations of ginseng polysaccharides injections(12.5μL/mL,25μL/mL,50μL/mL or 100μL/mL),and phosphate buffer saline was served as control.The invasive ability,transfer ability and protein expression of matrix metalloproteinases-2(MMP-2)and matrix metalloproteinases-9(MMP-9)were determined via transwell matrigel invasive test,scratch test and Western Blot,respectively.Results Ginseng polysaccharide injection on invasive ability and transfer ability in vitro of MCF-7 cell had significant role in inhibiting effect,and showed dose and time dependent.After ginseng polysaccharides injection treatment,the MMP-2 and MMP-9 protein expression significantly reduced,and showed dose and time dependent manner.Conclusion Ginseng polysaccharides injection is able to inhibit the invasion and transfer in MCF-7 cells.Moreover,the regulation of MMP-2 and MMP-9 expression may be involved in this effect.

Key words: Ginseng polysaccharides injection, Breast cancer, Metalloproteinases-2, Metalloproteinases-9

中图分类号:

R737.9

罗莹,黄晶. 人参多糖注射液对人乳腺癌MCF-7细胞侵袭迁移能力的影响及机制研究[J]. 实用肿瘤学杂志, 2015, 29(4): 333-336.

LUO Ying,HUANG Jing. The effect and mechanism of ginseng polysaccharides injective solution on invasive ability of human breast cancer MCF-7 cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(4): 333-336.

参考文献

1 Yoo DG,Kim MC,Park MK,et al.Protective effect of ginseng polysaccharides on influenza viral infection[J].PLoS One,2012,7(3):e33678.
2 Sun C,Chen Y,Li X,et al.Anti-hyperglycemic and anti-oxidative activities of ginseng polysaccharides in STZ-induced diabetic mice[J].Food & Function,2014,5(5):845-848.
3 Cheng H,Li S,Fan Y,et al.Comparative studies of the antiproliferative effects of ginseng polysaccharides on HT-29 human colon cancer cells[J].Medical Oncology,2011,28(1):175-181.
4 孙文洲,王晶,于丽波,等.熊果酸对卵巢癌细胞转移的抑制作用[J].实用肿瘤学杂志,2012,24(5):418-421.
5 吴剑锋,何晓东,许卫东,等.淫羊藿苷逆转耐甲氨蝶呤肺癌 A549 细胞转移表型[J].肿瘤,2009,29(12):1124-1128.
6 崔若凡,张宝刚,姬静,等.ARK5,MMP-2 和 MMP-9 在胃癌中的表达及与侵袭转移的关系[J].实用医学杂志,2012,28(7):1073-1076.
7 刘文娟,高鹏,王大海.人参多糖与他莫昔芬联合对人乳腺癌 MCF-7 细胞凋亡的影响及其机制研究[J].癌变.畸变.突变,2013,25(4):280-284.
8 Luan L,Zhao Y,Xu Z,et al.Diversin increases the proliferation and invasion ability of non-small-cell lung cancer cells via JNK pathway[J].Cancer Letters,2014,344(2):232-238.
9 Wang C,Gao C,Zhuang JL,et al.A combined approach identifies three mRNAs that are down-regulated by microRNA-29b and promote invasion ability in the breast cancer cell line MCF-7[J].Journal of Cancer Research and Clinical Oncology,2012,138(12):2127-2136.
10 Weigel M T,Krmer J,Schem C,et al.Differential expression of MMP-2,MMP-9 and PCNA in endometriosis and endometrial carcinoma[J].European Journal of Obstetrics & Gynecology and Reproductive Biology,2012,160(1):74-78.
11 Chen R,Cui J,Xu C,et al.The significance of MMP-9 over MMP-2 in HCC invasiveness and recurrence of hepatocellular carcinoma after curative resection[J].Annals of Surgical Oncology,2012,19(3):375-384.
12 Li X,Yang Z,Song W,et al.Overexpression of Bmi-1 contributes to the invasion and metastasis of hepatocellular carcinoma by increasing the expression of matrix metalloproteinase(MMP)2,MMP-9 and vascular endothelial growth factor via the PTEN/PI3K/Akt pathway[J].International Journal of Oncology,2013,43(3):793-802.
13 刘桃桃,申梦琴,王丹丹,等.三阴性乳腺癌中 MMP-9 及 E-cadherin 表达的意义[J].实用肿瘤学杂志,2014,28(1):19-23.
14 Bauvois B.New facets of matrix metalloproteinases MMP-2 and MMP-9 as cell surface transducers:outside-in signaling and relationship to tumor progression[J].Biochim Biophys Acta,2012,1825(1):29-36.
15 Langers AMJ,Verspaget HW,Hawinkels L,et al.MMP-2 and MMP-9 in normal mucosa are independently associated with outcome of colorectal cancer patients[J].British Journal of Cancer,2012,106(9):1495-1498.

[1]	王晓雅,肖斌,廖扬,唐荣芝,孙朝晖,李林海. CXCR家族蛋白在不同乳腺癌亚型中的表达及临床预后分析[J]. 实用肿瘤学杂志, 2019, 33(3): 206-210.
[2]	李烁,张楠楠,李亮亮,龙志平,尹慧慧,赵亚双,王帆. 外周血白细胞PDE4C基因甲基化及环境因素交互作用与乳腺癌发病的关系[J]. 实用肿瘤学杂志, 2019, 33(3): 233-238.
[3]	李嘉琪,栾瑾微,张,玉,李香兰. 763例三阴性乳腺癌临床病理特征及复发、转移影响因素分析[J]. 实用肿瘤学杂志, 2019, 33(3): 244-249.
[4]	陈艳波, 孔德嘉, 张金锋, 马志刚. 乳腺癌患者CYP2D6基因多态性与他莫昔芬代谢相关性研究[J]. 实用肿瘤学杂志, 2019, 33(2): 110-114.
[5]	孙江宏, 郝明珠, 姜丹, 国飞, 李晓梅. 乳腺炎、良性增生及乳腺癌FFDM鉴别诊断思路[J]. 实用肿瘤学杂志, 2019, 33(2): 139-141.
[6]	马志杰, 王帆, 赵亚双. 乳腺癌化疗患者生存质量及影响因素分析[J]. 实用肿瘤学杂志, 2019, 33(2): 149-154.
[7]	马质莹, 尚乃舰, 蔡妙田, 沈阳, 姜智允. 孕激素及雌激素受体阳性乳腺癌HER-2受体与钼靶影像学特征及临床病理学特征的相关性分析[J]. 实用肿瘤学杂志, 2019, 33(1): 47-51.
[8]	何煦, 叶尚勉, 董丹丹, 李科. HLA-G在乳腺癌中的表达及意义[J]. 实用肿瘤学杂志, 2018, 32(5): 410-414.
[9]	林荣杰, 尹航, 周云峰. ON123300对ER阳性人乳腺癌MCF-7细胞的影响及机制研究[J]. 实用肿瘤学杂志, 2018, 32(3): 193-197.
[10]	李林(综述), 李志高(审校). 二甲双胍与不同分子亚型乳腺癌的关系[J]. 实用肿瘤学杂志, 2018, 32(3): 267-270.
[11]	郑伟, 许守平, 庞达. 长链非编码RNA Linc00152在泛癌中表达、预后及功能研究[J]. 实用肿瘤学杂志, 2018, 32(1): 7-13.
[12]	殷玉莲, 张卫红, 周悦, 叶媚娜, 陈红风. ERα基因单核苷酸多态性与乳腺癌应用芳香化酶抑制剂引发骨丢失的相关性研究[J]. 实用肿瘤学杂志, 2018, 32(1): 14-18.
[13]	黎玉娇, 尚乃舰. 影像学评估乳腺癌新辅助化疗反应的研究进展[J]. 实用肿瘤学杂志, 2018, 32(1): 63-67.
[14]	陈琢, 孔艺然. 沉默UHRF1对乳腺癌细胞增殖和转移的影响[J]. 实用肿瘤学杂志, 2017, 31(6): 512-518.
[15]	李红阳, 孙亮, 姜醒, 苑光军, 刘艳. 蝙蝠葛碱在人乳腺癌MCF-7细胞对5氟尿嘧啶敏感性的研究[J]. 实用肿瘤学杂志, 2017, 31(5): 385-389.

人参多糖注射液对人乳腺癌MCF-7细胞侵袭迁移能力的影响及机制研究

The effect and mechanism of ginseng polysaccharides injective solution on invasive ability of human breast cancer MCF-7 cells

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价