蝙蝠葛碱在人乳腺癌MCF-7细胞对5氟尿嘧啶敏感性的研究

doi:10.11904/j.issn.1002-3070.2017.05.001

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (5): 385-389.doi: 10.11904/j.issn.1002-3070.2017.05.001

• 基础研究 • 下一篇

蝙蝠葛碱在人乳腺癌MCF-7细胞对5氟尿嘧啶敏感性的研究

李红阳¹, 孙亮², 姜醒³, 苑光军³, 刘艳¹

1.哈尔滨医科大学附属第四医院肿瘤内科(哈尔滨 150001);
2.哈尔滨医科大学人体解剖学教研室;
3.黑龙江中医药大学佳木斯学院

收稿日期:2017-01-17 出版日期:2017-10-28 发布日期:2017-10-24
通讯作者: 刘艳,E-mail:1953786691@qq.com
作者简介:李红阳,男,(1989-),硕士研究生,从事肿瘤内科的研究
基金资助:
黑龙江省自然科学基金面上项目(H201464);黑龙江省高校专项基金(003000166)

Dauricine enhances the sensitivity of 5-fluorouracil in human breast cancer MCF-7 cells

LI Hongyang¹, SUN Liang², JIANG Xing³, YUAN Guangjun³, LIU Yan¹

1.Department of Oncology,The Fourth Affiliated Hospital of Harbin Medical University,Harbin 150001,China;
2.Department of Human Anatomy,Harbin Medical University;
3.Jiamusi College of Heilongjiang University of Chinese Medicine

Received:2017-01-17 Online:2017-10-28 Published:2017-10-24

摘要/Abstract

摘要： 目的探讨人乳腺癌MCF-7细胞受蝙蝠葛碱(Dauricine,Dau)作用后,对5氟尿嘧啶(5-FU)敏感性的影响。方法分别以终浓度为2.5 μg/mL的蝙蝠葛碱、50 μg/mL的5-FU、2.5 μg/mL的蝙蝠葛碱和50 μg/mL的5-FU作用于人乳腺癌细胞MCF-7,运用MTT法检测细胞增殖活性,Transwell实验分析细胞的迁移,DAPI染色检测细胞的凋亡,Western blot法检测cyclinD1和Bcl-2蛋白的表达。结果 MTT实验结果显示联合使用阈下浓度的蝙蝠葛碱增强了5-FU对细胞增殖的抑制;Transwell实验表明联合应用阈下浓度的蝙蝠葛碱进一步加剧了5-FU对细胞迁移的抑制;DAPI染色说明联合应用阈下剂量的蝙蝠葛碱加强了5-FU对细胞凋亡的诱导,Western blot实验表明联合应用阈下浓度的蝙蝠葛碱进一步抑制了cyclinD1和Bcl-2蛋白的表达。结论蝙蝠葛碱可有效增强人乳腺癌MCF-7细胞对5-FU的敏感性。

关键词: 蝙蝠葛碱, 5氟尿嘧啶, 乳腺癌, MCF-7细胞

Abstract: Objective The objective of this study was to investigate the effect of dauricine on the sensitivity of 5-fluorouracil(5-FU)in human breast cancer MCF-7 cells.Methods MCF-7 cells were treated with 2.5 μg/mL of dauricine,50 μg/mL of 5-FU,or 2.5 μg/mL of dauricine with 50 μg/mL of 5-FU,the cell proliferation was measured by MTT assay.The cell migration was determined by Transwell assay;The cell apoptosis was detected by DAPI staining;The expression of cyclin D1 and Bcl-2 gene was examined by Western blot.Results The results showed that the combination of subthreshold concentration of dauricine enhanced the inhibitory effect of 5-FU on proliferation in MCF-7 cells.The combined use of subcutaneous concentration of dauricine further aggravated the inhibitory effect of 5-FU on cell migration.The combination of subcapsular dauricine enhanced the induction of apoptosis by 5-FU.The combination of dauricine with 5-FU could inhibit the expression of cyclin D1 and Bcl-2 protein in MCF-7 cells.Conclusion Dauricine can effectively enhance the sensitivity of 5-FU in human breast cancer MCF-7 cells.

Key words: Dauricine, 5-Fluorouracil, Breast cancer, MCF-7 cells

中图分类号:

R737.9

李红阳, 孙亮, 姜醒, 苑光军, 刘艳. 蝙蝠葛碱在人乳腺癌MCF-7细胞对5氟尿嘧啶敏感性的研究[J]. 实用肿瘤学杂志, 2017, 31(5): 385-389.

LI Hongyang, SUN Liang, JIANG Xing, YUAN Guangjun, LIU Yan. Dauricine enhances the sensitivity of 5-fluorouracil in human breast cancer MCF-7 cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(5): 385-389.

参考文献

1 Gradishar WJ,Anderson BO,Balassanian R,et al.NCCN guidelines insights breast cancer,version 1.2016[J].J Natl Compr Canc Netw,2015,13(12):1475-1485.
2 刘丽媛.女性乳腺癌危险因素及风险评估模型的流行病学研究[D].济南:山东大学,2015:1-45.
3 Gao J,Yan Q,Liu S,et al.Knockdown of EpCAM enhances the chemosensitivity of breast cancer cells to 5-fluorouracil by downregulating the antiapoptotic factor Bcl-2[J].PLoS One,2014,9(7):e102590.
4 Liu X,Liu Q,Wang D,et al.Validated liquid chromatography-tandem mass spectrometry method for quantitative determination of dauricine in human plasma and its application to pharmacokinetic study[J].J Chromatogr B,2010,878(15/16):1199-1203.
5 仲丽丽,张英博,白云,等.蝙蝠葛碱药理作用及其临床应用[J].辽宁中医药大学学报,2015,12(2):91-93.
6 张英博,周忠光,仲丽丽,等.蝙蝠葛碱抗肿瘤研究新进展[J].中国医学装备,2014,15(S2):370-371.
7 石玉生,张艳,王加志,等.蝙蝠葛碱抑制肺癌细胞QG-56增殖的实验研究[J].中医药信息,2010,27(3):115-116.
8 李铭,梁文杰,曾亚平,等.蝙蝠葛碱对人膀胱癌T-24细胞株生长增殖的抑制作用[J].第三军医大学学报,2006,28(18):1860-1863.
9 Yang Z,Li C,Wang X,et al.Dauricine induces apoptosis,inhibits proliferation and invasion through inhibiting NF-kappaB signaling pathway in colon cancer cells[J].J Cell Physiol,2010,225(1):266-275.
10 Finn,Richard S,John P,et al.The cyclin-dependent kinase 4/6 inhibitor palbociclib in combination with letrozole versus letrozole alone as first-line treatment of oestrogen receptor-positive,HER2-negative,advanced breast cancer(PALOMA-1/TRIO-18):a randomised phase 2 study[J].Lancet Oncology,2015,16(1):25-35.
11 Jahani M,Azadbakht M,Norooznezhad F,et al.l-arginine alters the effect of 5-fluorouracil on breast cancer cells in favor of apoptosis[J].Biomed Pharmacother,2017,88:114.
12 Mcdonnell TJ,Meyn RE,Robertson LE.Implications of apoptotic cell death regulation in cancer therapy[J].Semin Cancer Biol,1995,6(1):53.
13 Fantini MC,Pallone F.Cytokines:From gut inflammation to colorectal cancer[J].Curr Drug Targets,2008,9(5):375-380.
14 Karin M.Nuclear factor-kappaB in cancer development and progression[J].Nature,2006,441(7092):431-436.
15 Qian JQ.Cardiovascular pharmacological effects of bisbenzylisoquinoline alkaloid derivatives1[J].Acta Pharmacol Sin,2003,23(12):1086-1092.
16 Xia JS,Li Z,Dong JW,et al.Dauricine-induced changes in monophasic action potentials and effective refractory period of rabbit left ventricle in situ[J].Acta Pharmacol Sin,2002,23(4):371-375.

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蝙蝠葛碱在人乳腺癌MCF-7细胞对5氟尿嘧啶敏感性的研究

Dauricine enhances the sensitivity of 5-fluorouracil in human breast cancer MCF-7 cells

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