MicroRNA-150对上皮性卵巢癌细胞增殖、凋亡和侵袭转移能力的影响

doi:10.11904/j.issn.1002-3070.2018.03.003

实用肿瘤学杂志 ›› 2018, Vol. 32 ›› Issue (3): 208-213.doi: 10.11904/j.issn.1002-3070.2018.03.003

MicroRNA-150对上皮性卵巢癌细胞增殖、凋亡和侵袭转移能力的影响

李文辉¹, 吕博文¹, 钱钧², 苏丽菊³, 杨桐树¹, 钱景荣¹, 王杰¹

1.哈尔滨医科大学附属肿瘤医院(哈尔滨 150081);
2.哈尔滨医科大学;
3.哈尔滨市第一医院

收稿日期:2018-03-13 出版日期:2018-07-10 发布日期:2018-07-10
通讯作者: 李文辉,E-mail:iamliwh@sina.com
作者简介:李文辉,女,(1976-),博士,主任医师,从事临床检验诊断的研究
基金资助:
黑龙江省留学归国人员科学基金(编号:LC2015035)

Effect of microRNA-150 on proliferation,apoptosis,invasion and metastasis of epithelial ovarian cancer cells

LI Wenhui¹,LV Bowen¹,QIAN Jun²,SU Liju³,YANG Tongshu¹,QIAN Jingrong¹,WANG Jie¹

1.Harbin Medical University Cancer Hospital,Harbin 150081,China;
2.Harbin Medical University;
3.Harbin First Hospital

Received:2018-03-13 Online:2018-07-10 Published:2018-07-10

摘要/Abstract

摘要： 目的研究microRNA-150(miR-150)在人上皮性卵巢癌细胞中的表达情况,及其对人上皮性卵巢癌细胞增殖、凋亡和侵袭转移能力的影响。方法通过荧光实时定量PCR(qRT-PCR)检测各处理组细胞中的miR-150表达水平;利用MTT法、流式细胞技术、Transwell法探究miR-150的表达对上皮性卵巢癌细胞增殖、凋亡及侵袭转移能力的影响。结果与正常卵巢上皮细胞(T29)相比,上皮性卵巢癌细胞(A2780和OVCAR3)中miR-150表达显著降低(P＜0.01);转染miR-150mimic后,A2780和OVCAR3细胞中miR-150水平明显升高(P＜0.01);MTT试验显示,转染培养三天后,miR-150 mimic组细胞OD值(A2780:1.12±0.03;OVCAR3:1.91±0.03)较Blank组(A2780:2.35±0.09;OVCAR3:2.63±0.07)及miR-150 NC组(A2780:2.18±0.07;OVCAR3:2.43±0.11)明显降低(P＜0.01);细胞凋亡检测显示:miR-150 mimic组凋亡率(A2780:16.10±0.58%;OVCAR3:15.16±1.30%)较Blank组(A2780:10.07±0.66%;OVCAR3:3.81±0.24%)及miR-150 NC组(A2780:10.36±1.08%;OVCAR3:4.89±0.07%)明显升高(P＜0.01);Transwell试验显示:miR-150 mimic组穿膜细胞数(A2780:38.67±2.03;OVCAR3:28.67±2.03)较Blank组(A2780:76.30±7.45;OVCAR3:55.67±3.18)及miR-150 NC组(A2780:74.33±5.78;OVCAR3:56.33±3.84)明显减少(P＜0.01)。结论 miR-150在上皮性卵巢癌细胞中表达降低,可能是上皮性卵巢癌增殖、侵袭转移的机制之一;上调miR-150的表达可以抑制上皮性卵巢癌细胞增殖、促进细胞凋亡,并且降低上皮性卵巢癌细胞侵袭转移能力。

关键词: 上皮性卵巢癌细胞, miR-150, 增殖, 凋亡, 侵袭转移

Abstract: Objective The aim of this study was to investigate the expression of microRNA-150(miR-150)in human epithelial ovarian cancer cells and its effect on proliferation,apoptosis,invasion and metastasis of human epithelial ovarian cancer cells.Methods The expression level of miR-150 in cells from each treatment group was detected by Real-Time PCR(qRT-PCR);effects of proliferation,apoptosis,invasion and metastasis of epithelial ovarian cancer cells was investigated by MTT,flow cytometry,and transwell assays.Results Compared with normal ovarian epithelial cells(T29),the expression of miR-150 was significantly decreased in epithelial ovarian cancer cells(A2780 and OVCAR3)(P＜0.01); After transfection miR-150 mimic,the expression of miR-150 in A2780 and OVCAR3 cells was significantly increased(P＜0.01);After 3 d of transfection,the OD values of the miR-150 mimic group(A2780:1.12±0.03;OVCAR3:1.91±0.03)were lower than that in the blank group(A2780:2.35±0.09;OVCAR3:2.63±0.07)and the miR-150 NC group(A2780:2.18±0.07;OVCAR3:2.43±0.11)(P＜0.01);The apoptotic rate in the miR-150 mimic group(A2780:16.10±0.58%;OVCAR3:15.16±1.30%) were significantly increased when compared to the blank group(A2780:10.07±0.66%;OVCAR3:3.81±0.24%) and the miR-150 NC group(A2780:10.36±1.08%;OVCAR3:4.89±0.07%)(P＜0.01);The number of transmembrane cells in the miR-150 mimic group(A2780:38.67±2.03;OVCAR3:28.67±2.03)was higher than that in the blank group(A2780:76.30±7.45;OVCAR3:55.67±3.18)and the miR-150 NC group(A2780:74.33±5.78;OVCAR3:56.33±3.84)(P＜0.01).Conclusion The decreased expression of miR-150 in epithelial cancer cells may be one of the mechanisms of proliferation,invasion and metastasis of epithelial ovarian cancer.Up-regulation of miR-150 may inhibit the proliferation of epithelial ovarian cancer cells and promote apoptosis to reduce the abilities of invasion and metastasis in epithelial ovarian cancer cells.

Key words: Epithelial ovarian cancer, miR-150, Proliferation, Apoptosis, Invasion

中图分类号:

R737.31

李文辉, 吕博文, 钱钧, 苏丽菊, 杨桐树, 钱景荣, 王杰. MicroRNA-150对上皮性卵巢癌细胞增殖、凋亡和侵袭转移能力的影响[J]. 实用肿瘤学杂志, 2018, 32(3): 208-213.

LI Wenhui, LV Bowen, QIAN Jun, SU Liju, YANG Tongshu, QIAN Jingrong, WANG Jie. Effect of microRNA-150 on proliferation,apoptosis,invasion and metastasis of epithelial ovarian cancer cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(3): 208-213.

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MicroRNA-150对上皮性卵巢癌细胞增殖、凋亡和侵袭转移能力的影响

Effect of microRNA-150 on proliferation,apoptosis,invasion and metastasis of epithelial ovarian cancer cells

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