HLA-G在乳腺癌中的表达及意义

doi:10.11904/j.issn.1002-3070.2018.05.005

实用肿瘤学杂志 ›› 2018, Vol. 32 ›› Issue (5): 410-414.doi: 10.11904/j.issn.1002-3070.2018.05.005

HLA-G在乳腺癌中的表达及意义

何煦¹, 叶尚勉¹, 董丹丹², 李科²

1.四川省医学科学院;四川省人民医院中心实验室(成都 610072);
2.四川省医学科学院;四川省人民医院病理科

收稿日期:2018-06-15 出版日期:2018-10-28 发布日期:2018-10-23
通讯作者: 何煦,E-mail:123182096@qq.com
作者简介:何煦,女,(1974-),博士,副主任医师,从事肿瘤免疫学的研究。
基金资助:
四川省卫计委科研资助项目(编号:120106)

The expression of HLA-G in breast cancer and its significance

HE Xu¹, YE shangmian¹, DONG dandan², LI Ke²

1.Core Laboratory,Sichuan Academy of Medical Sciences,Sichuan Provincial People′s Hospital,Chengdu 610072,China;
2.Sichuan Academy of Medical Sciences and the department of pathology,Sichuan Provincial People′s Hospital

Received:2018-06-15 Online:2018-10-28 Published:2018-10-23

摘要/Abstract

摘要： 目的探讨乳腺癌患者组织中人类白细胞抗原G(Human leukocyte antigen,HLA-G)的表达及其在乳腺癌诊断和预后中的作用。方法分离纯化HLA-G蛋白,筛选出分泌抗HLA-G单克隆抗体的杂交瘤细胞株,得到抗HLA-G单克隆抗体。用免疫组织化学方法检测235例乳腺癌患者组织中HLA-G的表达,分析HLA-G的表达与各项临床病理指标、宿主免疫反应及生存率之间的关系。结果 HLA-G在乳腺癌患者的表达率为66%(155/235),HLA-G表达与肿瘤大小、临床TNM分期以及淋巴结转移有关。HLA-G阴性病人的生存率明显高于HLA-G阳性病人。结论乳腺癌组织中HLA-G有较高表达,HLA-G的表达与宿主免疫反应及乳腺癌的预后有关,HLA-G的表达可能是影响乳腺癌病人预后的独立因素。

关键词: 人类白细胞抗原G(HLA-G), 乳腺癌, 免疫组织化学

Abstract: Objective The Objective of this study was to investigate the expression of human leukocye antigen-G(HLA-G)in breast cancer patients and its role in the diagnosis and prognosis of breast cancer.Methods HLA-G protein was isolated and purified,and hybridoma cell lines secreting anti-HLA-G monoclonal antibody were screened to obtain anti-HLA-G monoclonal antibody.The expression of HLA-G in 235 primary breast cancer tissues was detected by immunohistochemistry.The relationship amongst HLA-G expression with various clinicopathological parameters,host immune response & survival rate was analyzed.Results The expression rate of HLA-G in breast cancer patients was 66%(155/235).The expression of HLA-G was related to tumor size,clinical TNM stage and lymph node metastasis.The survival rate of HLA-G negative patients was significantly higher than that of HLA-G positive patients.Conclusion HLA-G is highly expressed in breast cancer tissues.The expression of HLA-G is related to host immune response and prognosis of breast cancer.HLA-G is an independent factor affecting the prognosis of breast cancer patients.

Key words: Human leukocyte antigen-G(HLA-G), Breast cancer, Immunohistochemistry

中图分类号:

R737.9

何煦, 叶尚勉, 董丹丹, 李科. HLA-G在乳腺癌中的表达及意义[J]. 实用肿瘤学杂志, 2018, 32(5): 410-414.

HE Xu, YE shangmian, DONG dandan, LI Ke. The expression of HLA-G in breast cancer and its significance[J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(5): 410-414.

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HLA-G在乳腺癌中的表达及意义

The expression of HLA-G in breast cancer and its significance

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