基于UPLC-TOF/MS的小细胞肺癌患者血清代谢组学研究

doi:10.11904/j.issn.1002-3070.2019.03.002

实用肿瘤学杂志 ›› 2019, Vol. 33 ›› Issue (3): 200-205.doi: 10.11904/j.issn.1002-3070.2019.03.002

基于UPLC-TOF/MS的小细胞肺癌患者血清代谢组学研究

张佳文¹,于雁²,张薇¹,曹京燕²,罗雯¹,尚利华²

1.哈尔滨医科大学附属第一医院呼吸内科(哈尔滨 150001);
2.哈尔滨医科大学附属肿瘤医院内六科

收稿日期:2019-03-05 修回日期:2019-04-23 出版日期:2019-06-20 发布日期:2019-06-18
通讯作者: 尚利华,E-mail:shanglhsmile@163.com
作者简介:张佳文,女,(1989-),博士,住院医师,从事呼吸内科危重症及呼吸系统肿瘤诊疗工作的研究。
基金资助:
黑龙江省卫生厅科研课题(编号:666)

Serum metabolomics of small cell lung cancer patients based on UPLC-TOF/MS

ZHANG Jiawen¹,YU Yan²,ZHANG Wei¹,CAO Jingyan²,LUO Wen¹,SHANG Lihua²

1.Department of Respiratory,The First Clinical Hospital of Harbin Medical University,Harbin 150001,China;
2.Department of Respiratory Oncology,Harbin Medical University Cancer Hospital

Received:2019-03-05 Revised:2019-04-23 Online:2019-06-20 Published:2019-06-18

摘要/Abstract

摘要： 目的探索小细胞肺癌患者与健康人血清代谢组学差异,为小细胞肺癌的鉴定与分期寻找血清潜在生物标志物。方法利用超高效液相色谱-飞行时间质谱联用仪(UPLC-TOF/MS)建立小细胞肺癌血清代谢图谱,采用EZinfo2.0软件进行主成分分析(Principal component analysis,PCA)、正交隐变量投影判别分析(Orthogonal partial least squares discriminant analysis,OPLS-DA)分析病例组和正常对照组之间的代谢差异。通过聚类分析并利用HMDB和METLIN数据库搜索差异物的精确质荷比,对一些具有显著性差异的物质进行初步的成分鉴别。结果通过质谱分析和数据库检索筛选并鉴定出溶血性磷脂酰胆碱等10种差异性代谢产物,不同分期的小细胞肺癌患者轮廓分析存在甘氨胆酸等10种差异性物质。结论小细胞肺癌患者与健康对照人群在血清代谢水平上具有明显差异,差异代谢物的发现为小细胞肺癌的鉴定以及分期寻找潜在标志物提供实验依据。

关键词: 小细胞肺癌, 代谢组学, 肿瘤标志物, UPLC-TOF/MS

Abstract: Objective The Objective of this study was to explore the differences of serum metabolomics between small cell lung cancer(SCLC)patients and healthy volunteers,and to discover serum potential biomarkers for identification and small cell lung cancer staging.Methods Ultra-performance liquid chromatography time of flight mass spectrometry(UPLS-TOF/MS)was used to establish the serum metabolic profile of SCLC.Principal Component Analysis(PCA)and orthogonal hidden variables were analyzed by the EZinfo2.0 software.Orthogonal Partial Least Squares Discriminant Analysis(OPLS-DA)was used to analyze the metabolic differences between the case and normal control groups.Through cluster analysis using HMDB and METLIN database to search for the exact mass-to-charge ratio of the difference,preliminary identification of some substances with significant differences was carried out.Results Ten differential metabolites such as lysophosphatidylcholine between patients and control groups were screened and identified by mass spectrometry and database search.There were 10 different metabolites such as glycocholic acid in the contour analysis of SCLC patients with different stages.Conclusion There is a significant difference in serum metabolism between SCLC patients and healthy controls.The discovery of differential metabolites provides experimental evidence for the identification of small cell lung cancer and potential markers of staging.

Key words: Small lung cancer, Metabolites, Biomarker, UPLC-TOF/MS

中图分类号:

R734.2

张佳文,于雁,张薇,曹京燕,罗雯,尚利华. 基于UPLC-TOF/MS的小细胞肺癌患者血清代谢组学研究[J]. 实用肿瘤学杂志, 2019, 33(3): 200-205.

ZHANG Jiawen,YU Yan,ZHANG Wei,CAO Jingyan,LUO Wen,SHANG Lihua. Serum metabolomics of small cell lung cancer patients based on UPLC-TOF/MS[J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(3): 200-205.

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基于UPLC-TOF/MS的小细胞肺癌患者血清代谢组学研究

Serum metabolomics of small cell lung cancer patients based on UPLC-TOF/MS

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