MAGEA4和EB1蛋白在肺癌组织中的表达及其与临床病理特征及预后的相关性分析

doi:10.11904/j.issn.1002-3070.2019.04.009

摘要/Abstract

摘要： 目的探讨MAGEA4和EB1蛋白在肺癌组织中的表达及其与临床病理特征及预后的相关性。方法选取2010年3月—2012年5月在我院胸外科行手术切除并经病理证实的肺癌患者136例为研究对象,采用实时荧光逆转录法及免疫组织化学法测定MAGEA4、EB1 mRNA及蛋白的表达水平,采用χ²检验及Cox回归分析探讨其与患者临床病理特征及预后的相关性。结果肺癌组织中MAGEA4、EB1 mRNA表达水平高于癌旁组织(P＜0.05);肺癌组织中MAGEA4、EB1阳性率高于癌旁组织(P＜0.05);肺癌组织中MAGEA4、EB1蛋白表达高于癌旁组织(P＜0.05);MAGEA4、EB1蛋白阳性表达率与年龄无关(P＞0.05),与肿瘤最大径、病理学分级、TNM分期、浸润深度、淋巴血管间隙浸润、淋巴结转移、复发有关(P＜0.05)。MAGEA4、EB1阴性组3年生存率及总生存期均明显高于MAGEA4、EB1阳性组(P＜0.05)。淋巴血管间隙浸润、淋巴结转移、MAGEA4阳性、EB阳性是肺癌患者预后的独立危险因素(P＜0.05)。结论 MAGEA4和EB1蛋白在肺癌组织中阳性表达率增高,其高表达可能与肺癌的发生发展相关;MAGEA4、EB1蛋白阴性表达的肺癌患者能获得较好的预后。

关键词: 黑素瘤抗原家族A4, 人末端结合蛋白1, 肺癌, 临床病理特征, 预后

Abstract: Objective The aim of this study was to investigate the expression of MAGEA4 and EB1 proteins in lung cancer tissues and their correlation with clinicopathological features and prognosis.Methods A total of 136 patients with lung cancer in our hospital were enrolled.The expression levels of MAGEA4 and EB1 at levels of mRNA and protein were measured by real-time fluorescence reverse transcription and immunohistochemistry.The correlation between MAGEA4 and EB1 expression and clinical pathological features,and prognosis were analyzed by χ² test and Cox regression analysis.Results The expression of MAGEA4 and EB1 mRNA in lung cancer tissues was higher than those in adjacent tissues(P＜0.05).The positive rates of MAGEA4 and EB1 in lung cancer tissues were higher than those in adjacent tissues(P＜0.05).The expression of MAGEA4 and EB1 proteins in lung cancer tissues was higher than those in adjacent tissues(P＜0.05).The positive rates of MAGEA4 and EB1 proteins were not significantly correlated with age(P＞0.05),but they were related to the maximum diameter,pathological grade,TNM stage,infiltration depth,lymphatic vascular infiltration,lymph node metastasis and recurrence(P＜0.05).The 3-year survival rate and total survival time of MAGEA4 and EB1 negative group were significantly higher than those of MAGEA4 and EB1 positive group(P＜0.05).Lymphatic vascular infiltration,lymph node metastasis,MAGEA4 positive and EB positive were independent risk factors for prognosis of patients with lung cancer(P＜0.05).Conclusion The positive expression rates of MAGEA4 and EB1 proteins in lung cancer tissues are increased,and their high expression may be related to the occurrence and development of lung cancer.Lung cancer patients with negative expression of MAGEA4 and EB1 proteins can obtain better prognosis.

Key words: MAGEA4, EB1, Lung cancer, Clinico pathological features, Prognosis

中图分类号:

R734.2

刘金涛, 高玲, 周会会. MAGEA4和EB1蛋白在肺癌组织中的表达及其与临床病理特征及预后的相关性分析[J]. 实用肿瘤学杂志, 2019, 33(4): 334-339.

LIU Jintao, GAO Ling, ZHOU Huiui. Expression of MAGEA4 and EB1 proteins in lung cancer tissues and their correlation with clinicopathological features and prognosis[J]. Journal of Practical Oncology, 2019, 33(4): 334-339.

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