Abstract: Objective To investigate the relation between the expression of matrix metalloproteinase 2(MMP-2),matrix metalloproteinase 14(MMP-14)in breast cancer and tumor size,differentiation and lymph node metastases.Methods Sixtyeight cases of breast cancer and twentyfour cases of breast benign disease were studied by PV Immunohistochemical staining with anti MMP-2,MMP-14 monoclonal antibodies.Results 1.The expression rates of MMP-2 and MMP-14 were 55.9 %(38/ 68)and 58.8 %(40/68)in breast cancers respectively,they were 25.0%(6/24) and 16.7%(4/24)respectively in breast benign disease.The expression rates were significantly higher than those in breast benign disease(P＜0.05);2.Expression of MMP-2 and MMP-14 in breast cancers were positive correlated with axillary lymph node metastases and tumor size(P＜0.05).Conclusion The expression of MMP-2 and MMP-14 closely correlated to lymph node metastasis and tumor size,they can act as prognostic markers of invasion and metastasis for patients with breast cancer.