Abstract: Objective To investigate differential expression of HMGA2 and Smad4 in gastric carcinoma and its significance.To investigate association between HMGA2 and epithelial mesenchymal transitions.Methods Tissue samples from 80 gastric carcinoma tissues and 20 adjacent tissues were examined by immunohistochemistry for the expressions of HMGA2, Smad4, E-cadherin and vimentin.Results The positive expressions of HMGA2 and vimentin were 41.25%(55/80)and 67.5%(54/80), respectively.It is higher than that of adjacent tissues(P＜0.05).The positive expression of Smad4 and E-cad were 17.5%(14/80)and 26.25%(21/80)respectively, which is lower than that of adjacent tissues(P＜0.05).HMGA2 and vimentin expression in the gastric carcinoma was positive correlation;Smad4, E-cadherin and HMGA2 in the gastric carcinoma was negative correlation.The expression of HMGA2 was not related to the age and sexual, but it was correlated with differentiation, TNM stage and lymph node metastasis(P＜0.05).The expression of Smad4 was correlated with infiltration, TNM stage and lymph node metastasis(P＜0.05).Conclusion The upregulated expression of HMGA2, vimentin and the down regulated expressions of Smad4 and E-cadherin will promote the growth and the lymph node metastasis of gastric cancinoma in some degree.HMGA2 and Smad4 can be regarded as very important biologic markers in the gastric cancer diagnosis and prognosis.