Abstract: Objecive To explore the significance of expression of tumor-associated macrophages(TAM)in node-negative breast cancer.Methods Immunohistochemical staining was used to analyze TAMs expression levels in patients with breast cancer and compared these data with the clinicopathological features of these patients.Results Breast cancer patients with TAMs high-infiltration had significantly lower DFS and 5-year survival rates than patients with TAMs low-infiltration.For patients with negative axillary lymph node metastasis, high-infiltration of TAMs indicated markedly poorer survival rate than low-infiltration arm.Multivariate analysis further confirmed that increased density of TAMs was an independent prognostic factor for patients with breast cancer.Conclusion High infiltration of TAM indicated poor survival rate for patients with node-negative breast cancer.The infiltration of TAMs is a new and important risk factor for node-negative breast cancer recurrence.