实用肿瘤学杂志 ›› 2023, Vol. 37 ›› Issue (4): 337-343.doi: 10.11904/j.issn.1002-3070.2023.04.007

• 临床研究 • 上一篇    下一篇

骨肉瘤中Galectin-9和CD8的表达与临床病理特征及预后相关性分析

颜春辉, 喻玲, 何文源, 崔文志, 王卓才, 闫广宁   

  1. 中国人民解放军南部战区总医院病理科(广州 510010)
  • 收稿日期:2022-08-16 修回日期:2023-02-01 出版日期:2023-08-28 发布日期:2023-12-12
  • 通讯作者: 闫广宁,E-mail:cawafrog@hotmail.com
  • 作者简介:颜春辉,女,(1991-),硕士,主治医师,从事肿瘤免疫方面的研究。
  • 基金资助:
    国家自然科学青年基金(编号:81802962)

Expression of Galectin-9 and CD8 in osteosarcoma and their correlation with clinical pathological characteristics and prognosis

YAN Chunhui, YU Ling, HE Wenyuan, CUI Wenzhi, WANG Zhuocai, YAN Guangning   

  1. Department of Pathology, General Hospital of Southern Theatre Command, Guangzhou 510010, China
  • Received:2022-08-16 Revised:2023-02-01 Online:2023-08-28 Published:2023-12-12

摘要: 目的 检测骨肉瘤组织中Galectin-9(Gal-9)和CD8的表达,分析其表达水平与骨肉瘤临床病理特征的关系,探讨对预后的影响。方法 本研究纳入2006—2016年期间初诊初治行手术切除并术后病理证实为骨肉瘤患者58例,并收集其临床及病理资料。检测Gal-9及CD8的表达并分析对骨肉瘤患者的无进展生存期(DFS)和总生存期(OS)的影响。结果 免疫组化结果显示Gal-9低表达47例(81.0%),高表达11例(19.0%);CD8低表达28例(48.3%),高表达30例(51.7%)。Gal-9高表达组相比低表达组,肿瘤直径显著增大(P<0.05);CD8低表达相比高表达组,DFS显著缩短(P<0.05)。Gal-9低表达且CD8高表达组(n=24)女性比例更高,肿瘤直径较小,DFS和OS均更长(P<0.05)。多因素Cox回归分析显示,Gal-9低表达且CD8高表达是影响骨肉瘤患者DFS和OS的独立因素(P<0.05)。结论 骨肉瘤患者Gal-9低表达且CD8高表达提示预后较好,可以为骨肉瘤患者预后分层和免疫治疗靶点选择提供依据。

关键词: 骨肉瘤, Galectin-9, CD8, 预后

Abstract: Objective The objective of this study was to detect the expressions of Galectin-9(Gal-9)and CD8 in osteosarcoma tissues, analyze the relationship between their expression and the clinical pathological features of osteosarcoma, and explore the impact on prognosis. Methods This study included 58 patients with initial diagnosis, surgical resection, and postoperative pathological confirmation of osteosarcoma from 2006 to 2016, and collected their clinical and pathological data. The expression of Gal-9 and CD8 was detected and the disease-free survival(DFS)and overall survival(OS)of osteosarcoma patients were statistically analyzed. Results The immunohistochemical results revealed that Gal-9 was low expressed in 47 cases(81.0%)and highly expressed in 11 cases(19.0%);CD8 was low expressed in 28 cases(48.3%)and highly expressed in 30 cases(51.7%). The high expression group of Gal-9 showed a significantly increase in tumor diameter compared to the low expression group(P<0.05). Compared with the high expression group, the low expression group of CD8 significantly shortened DFS(P<0.05). The group with low expression of Gal-9 and high expression of CD8(n=24)had a significantly higher proportion of women, shorter tumor diameter, and longer DFS and OS(P<0.05). Multivariate Cox analysis showed that low expression of Gal-9 and high expression of CD8 were independent prognostic factors affecting DFS and OS in patients with osteosarcoma(P<0.05). Conclusion Low expression of Gal-9 and high expression of CD8 in osteosarcoma patients indicate a better prognosis, which can provide a basis for prognostic stratification and selection of immunotherapy targets in osteosarcoma patients.

Key words: Osteosarcoma, Galectin-9, CD8, Prognosis

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